Cited 12 times in
Functional characterization of a bioengineered liver after heterotopic implantation in pigs
DC Field | Value | Language |
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dc.contributor.author | 주동진 | - |
dc.date.accessioned | 2022-09-14T01:39:30Z | - |
dc.date.available | 2022-09-14T01:39:30Z | - |
dc.date.issued | 2021-10 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/190558 | - |
dc.description.abstract | Organ bioengineering offers a promising solution to the persistent shortage of donor organs. However, the progression of this technology toward clinical use has been hindered by the challenges of reconstituting a functional vascular network, directing the engraftment of specific functional cell types, and defining appropriate culture conditions to concurrently support the health and phenotypic stability of diverse cell lineages. We previously demonstrated the ability to functionally reendothelialize the vasculature of a clinically scaled decellularized liver scaffold with human umbilical vein endothelial cells (HUVECs) and to sustain continuous perfusion in a large animal recovery model. We now report a method for seeding and engrafting primary porcine hepatocytes into a bioengineered liver (BEL) scaffold previously reendothelialized with HUVECs. The resulting BELs were competent for albumin production, ammonia detoxification and urea synthesis, indicating the presence of a functional hepatocyte compartment. BELs additionally slowed ammonia accumulation during in vivo perfusion in a porcine model of surgically induced acute liver failure. Following explant of the graft, BEL parenchyma showed maintenance of canonical endothelial and hepatocyte markers. Taken together, these results support the feasibility of engineering a clinically scaled functional BEL and establish a platform for optimizing the seeding and engraftment of additional liver specific cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group UK | - |
dc.relation.isPartOf | COMMUNICATIONS BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Hepatocytes / transplantation | - |
dc.subject.MESH | Human Umbilical Vein Endothelial Cells / transplantation | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Liver / surgery | - |
dc.subject.MESH | Liver Failure, Acute / surgery | - |
dc.subject.MESH | Liver Transplantation / methods* | - |
dc.subject.MESH | Perfusion | - |
dc.subject.MESH | Sus scrofa / surgery | - |
dc.subject.MESH | Tissue Engineering / methods* | - |
dc.title | Functional characterization of a bioengineered liver after heterotopic implantation in pigs | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학교실) | - |
dc.contributor.googleauthor | Brett D Anderson | - |
dc.contributor.googleauthor | Erek D Nelson | - |
dc.contributor.googleauthor | DongJin Joo | - |
dc.contributor.googleauthor | Bruce P Amiot | - |
dc.contributor.googleauthor | Aleksandr A Katane | - |
dc.contributor.googleauthor | Alyssa Mendenhall | - |
dc.contributor.googleauthor | Benjamin G Steiner | - |
dc.contributor.googleauthor | Aron R Stumbras | - |
dc.contributor.googleauthor | Victoria L Nelson | - |
dc.contributor.googleauthor | R Noelle Palumbo | - |
dc.contributor.googleauthor | Thomas W Gilbert | - |
dc.contributor.googleauthor | Dominique S Davidow | - |
dc.contributor.googleauthor | Jeffrey J Ross | - |
dc.contributor.googleauthor | Scott L Nyberg | - |
dc.identifier.doi | 10.1038/s42003-021-02665-2 | - |
dc.contributor.localId | A03948 | - |
dc.relation.journalcode | J03836 | - |
dc.identifier.eissn | 2399-3642 | - |
dc.identifier.pmid | 34620986 | - |
dc.contributor.alternativeName | Joo, Dong Jin | - |
dc.contributor.affiliatedAuthor | 주동진 | - |
dc.citation.volume | 4 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1157 | - |
dc.identifier.bibliographicCitation | COMMUNICATIONS BIOLOGY, Vol.4(1) : 1157, 2021-10 | - |
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