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Detection of Infectious Viruses Using CRISPR-Cas12-Based Assay
DC Field | Value | Language |
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dc.contributor.author | 용동은 | - |
dc.date.accessioned | 2022-09-14T01:34:28Z | - |
dc.date.available | 2022-09-14T01:34:28Z | - |
dc.date.issued | 2021-09 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/190525 | - |
dc.description.abstract | The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease-19 (COVID-19), has severely influenced public health and economics. For the detection of SARS-CoV-2, clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein (Cas)-based assays have been emerged because of their simplicity, sensitivity, specificity, and wide applicability. Herein, we have developed a CRISPR-Cas12-based assay for the detection of SARS-CoV-2. In the assay, the target amplicons are produced by isothermal reverse transcription recombinase polymerase amplification (RT-RPA) and recognized by a CRISPR-Cas12a/guide RNA (gRNA) complex that is coupled with the collateral cleavage activity of fluorophore-tagged probes, allowing either a fluorescent measurement or naked-eye detection on a lateral flow paper strip. This assay enables the sensitive detection of SARS-CoV-2 at a low concentration of 10 copies per sample. Moreover, the reliability of the method is verified by using nasal swabs and sputum of COVID-19 patients. We also proved that the current assay can be applied to other viruses, such as Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus (SARS-CoV), with no major changes to the basic scheme of testing. It is anticipated that the CRISPR-Cas12-based assay has the potential to serve as a point-of-care testing (POCT) tool for a wide range of infectious viruses. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | MDPI Pub. | - |
dc.relation.isPartOf | BIOSENSORS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Detection of Infectious Viruses Using CRISPR-Cas12-Based Assay | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Chandana S Talwar | - |
dc.contributor.googleauthor | Kwang-Hyun Park | - |
dc.contributor.googleauthor | Woo-Chan Ahn | - |
dc.contributor.googleauthor | Yong-Sam Kim | - |
dc.contributor.googleauthor | Oh Seok Kwon | - |
dc.contributor.googleauthor | Dongeun Yong | - |
dc.contributor.googleauthor | Taejoon Kang | - |
dc.contributor.googleauthor | Euijeon Woo | - |
dc.identifier.doi | 10.3390/bios11090301 | - |
dc.contributor.localId | A02423 | - |
dc.relation.journalcode | J04064 | - |
dc.identifier.eissn | 2079-6374 | - |
dc.identifier.pmid | 34562891 | - |
dc.subject.keyword | COVID-19 | - |
dc.subject.keyword | CRISPR-Cas12 | - |
dc.subject.keyword | SARS-CoV-2 | - |
dc.subject.keyword | infectious disease | - |
dc.subject.keyword | virus | - |
dc.contributor.alternativeName | Yong, Dong Eun | - |
dc.contributor.affiliatedAuthor | 용동은 | - |
dc.citation.volume | 11 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 301 | - |
dc.identifier.bibliographicCitation | BIOSENSORS, Vol.11(9) : 301, 2021-09 | - |
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