Cited 2 times in
Sphere-Forming Culture for Expanding Genetically Distinct Patient-Derived Glioma Stem Cells by Cellular Growth Rate Screening
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이혜원 | - |
dc.date.accessioned | 2022-09-06T06:08:46Z | - |
dc.date.available | 2022-09-06T06:08:46Z | - |
dc.date.issued | 2020-03 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/190208 | - |
dc.description.abstract | Diffusely infiltrating gliomas (DIGs) are difficult to completely resect and are associated with a high rate of tumor relapse and progression from low- to high-grade glioma. In particular, optimized short-term culture-enriching patient-derived glioma stem cells (GSCs) are essential for customizing the therapeutic strategy based on clinically feasible in vitro drug screening for a wide range of DIGs, owing to the high inter-tumoral heterogeneity. Herein, we constructed a novel high-throughput culture condition screening platform called 'GFSCAN', which evaluated the cellular growth rates of GSCs for each DIG sample in 132 serum-free combinations, using 13 previously reported growth factors closely associated with glioma aggressiveness. In total, 72 patient-derived GSCs with available genomic profiles were tested in GFSCAN to explore the association between cellular growth rates in specific growth factor combinations and genomic/molecular backgrounds, including isocitrate dehydrogenase 1 (IDH1) mutation, chromosome arm 1p and 19q co-deletion, ATRX chromatin remodeler alteration, and transcriptional subtype. GSCs were clustered according to the dependency on epidermal growth factor and basic fibroblast growth factor (E&F), and isocitrate dehydrogenase 1 (IDH1) wild-type GSCs showed higher E&F dependencies than IDH1 mutant GSCs. More importantly, we elucidated optimal combinations for IDH1 mutant glioblastoma and lower grade glioma GSCs with low dependencies on E&F, which could be an aid in clinical decision-making for these DIGs. Thus, we demonstrated the utility of GFSCAN in personalizing in vitro cultivation to nominate personalized therapeutic options, in a clinically relevant time frame, for individual DIG patients, where standard clinical options have been exhausted. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | CANCERS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Sphere-Forming Culture for Expanding Genetically Distinct Patient-Derived Glioma Stem Cells by Cellular Growth Rate Screening | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Hospital Medicine (입원의학과) | - |
dc.contributor.googleauthor | Kayoung Shin | - |
dc.contributor.googleauthor | Hyemi Shin | - |
dc.contributor.googleauthor | Hee Jin Cho | - |
dc.contributor.googleauthor | Hyunju Kang | - |
dc.contributor.googleauthor | Jin-Ku Lee | - |
dc.contributor.googleauthor | Yun Jee Seo | - |
dc.contributor.googleauthor | Yong Jae Shin | - |
dc.contributor.googleauthor | Donggeon Kim | - |
dc.contributor.googleauthor | Harim Koo | - |
dc.contributor.googleauthor | Doo-Sik Kong | - |
dc.contributor.googleauthor | Ho Jun Seol | - |
dc.contributor.googleauthor | Jung-Il Lee | - |
dc.contributor.googleauthor | Hye Won Lee | - |
dc.contributor.googleauthor | Do-Hyun Nam | - |
dc.identifier.doi | 10.3390/cancers12030549 | - |
dc.contributor.localId | A05913 | - |
dc.relation.journalcode | J03449 | - |
dc.identifier.eissn | 2072-6694 | - |
dc.identifier.pmid | 32120790 | - |
dc.subject.keyword | use infiltrating glioma | - |
dc.subject.keyword | personalized medicine | - |
dc.subject.keyword | glioma stem cells | - |
dc.subject.keyword | short-term cultivation screening platform | - |
dc.subject.keyword | growth factor | - |
dc.subject.keyword | genomic profiling | - |
dc.contributor.alternativeName | Lee, Hye Won | - |
dc.contributor.affiliatedAuthor | 이혜원 | - |
dc.citation.volume | 12 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 549 | - |
dc.identifier.bibliographicCitation | CANCERS, Vol.12(3) : 549, 2020-03 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.