Cited 4 times in
Newly synthesized peptide, Ara-27, exhibits significant improvement in cell-penetrating ability compared to conventional peptides
DC Field | Value | Language |
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dc.contributor.author | 박정윤 | - |
dc.date.accessioned | 2022-09-02T01:09:28Z | - |
dc.date.available | 2022-09-02T01:09:28Z | - |
dc.date.issued | 2020-09 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/190012 | - |
dc.description.abstract | Cell-penetrating peptides (CPPs) are short amino acid sequences known to act as a vehicle for enhancing the intracellular translocating efficiency of extracellular molecules. Although many groups have attempted to develop peptides with high cell-penetrating efficiencies, very few have demonstrated efficient cellular uptake of CPPs at low concentrations. Here, we describe a newly synthesized peptide derived fromArabidopsis, Ara-27, which exhibits significant improvement in cell-penetrating efficiency compared to existing CPPs. The cell-penetrating efficiency of Ara-27 was compared with the commonly used Tat-protein transduction domain (Tat-PTD) and membrane translocating sequence (MTS) in human dermal fibroblast (HDF) and human dental pulp stem cells (hDPSC). Cell-penetrating efficiency of fluorescein isothiocyanate (FITC)-labeled CPPs were assessed by flow cytometry and visualized by confocal microscopy. Flow cytometric analysis revealed >99% cell-penetrating efficiency for 2 mu M Ara-27 in both HDF and hDPSC. In contrast, 2 mu M Tat-PTD and MTS showed <10% cell-penetrating efficiency in both cells. In support, relative fluorescence intensities of FITC-labeled Ara-27 were around 8 to 22 times higher than those of Tat-PTD and MTS in both cells. Confocal analysis revealed internalization of 0.2 and 2 mu M Ara-27 in both human cells, which was not observed for Tat-PTD and MTS at either concentration. In conclusion, this study describes a novel CPP, Ara-27, which exhibit significant improvement in intracellular uptake compared to conventional CPPs, without affecting cell viability. Thus, development of Ara-27 based peptides may lead to improved delivery of functional cargo such as small molecules, siRNA, and drugs for in vivo studies. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Wiley-Blackwell | - |
dc.relation.isPartOf | BIOTECHNOLOGY PROGRESS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Arabidopsis Proteins / chemistry | - |
dc.subject.MESH | Arabidopsis Proteins / metabolism | - |
dc.subject.MESH | Cell-Penetrating Peptides* / chemistry | - |
dc.subject.MESH | Cell-Penetrating Peptides* / metabolism | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Drug Delivery Systems / methods* | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Microscopy, Confocal | - |
dc.subject.MESH | Nanoparticles* / chemistry | - |
dc.subject.MESH | Nanoparticles* / metabolism | - |
dc.subject.MESH | Zinc / chemistry | - |
dc.title | Newly synthesized peptide, Ara-27, exhibits significant improvement in cell-penetrating ability compared to conventional peptides | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurosurgery (신경외과학교실) | - |
dc.contributor.googleauthor | Sol Min | - |
dc.contributor.googleauthor | Kichul Kim | - |
dc.contributor.googleauthor | Seockmo Ku | - |
dc.contributor.googleauthor | Jeong-Yoon Park | - |
dc.contributor.googleauthor | Jeongmin Seo | - |
dc.contributor.googleauthor | Sangho Roh | - |
dc.identifier.doi | 10.1002/btpr.3014 | - |
dc.contributor.localId | A01650 | - |
dc.relation.journalcode | J03120 | - |
dc.identifier.eissn | 1520-6033 | - |
dc.identifier.pmid | 32374475 | - |
dc.identifier.url | https://aiche.onlinelibrary.wiley.com/doi/10.1002/btpr.3014 | - |
dc.subject.keyword | drug delivery | - |
dc.subject.keyword | nanocarrier | - |
dc.subject.keyword | nanoparticle | - |
dc.subject.keyword | zinc knuckle | - |
dc.contributor.alternativeName | Park, Jeong Yoon | - |
dc.contributor.affiliatedAuthor | 박정윤 | - |
dc.citation.volume | 36 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | e3014 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY PROGRESS, Vol.36(5) : e3014, 2020-09 | - |
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