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Simultaneous dual-targeted monitoring of breast cancer circulating miRNA via surface-enhanced Raman spectroscopy

Authors
 Jinyoung Kim  ;  Joowon Park  ;  Jisun Ki  ;  Hyun Wook Rho  ;  Yong-Min Huh  ;  Eunjung Kim  ;  Hye Young Son  ;  Seungjoo Haam 
Citation
 BIOSENSORS & BIOELECTRONICS, Vol.207 : 114143, 2022-07 
Journal Title
BIOSENSORS & BIOELECTRONICS
ISSN
 0956-5663 
Issue Date
2022-07
MeSH
Biosensing Techniques* / methods ; Breast Neoplasms* / diagnosis ; Circulating MicroRNA* ; Female ; Gold / chemistry ; Humans ; Metal Nanoparticles* / chemistry ; MicroRNAs* / analysis ; MicroRNAs* / genetics ; Silver / chemistry ; Spectrum Analysis, Raman / methods
Keywords
Breast cancer ; Liquid biopsy ; Molecular diagnosis ; Surface enhanced Raman scattering ; Urinary microRNA
Abstract
Breast cancer is one of the most common cancers globally. Because the 5-year survival rate of breast cancer greatly increases when treated in its initial stage, the importance of early detection has been increasing. Herein, one-spot multiple breast cancer circulating microRNA (miRNA) detection via surface-enhanced Raman spectroscopy (SERS) with seed-mediated grown Ag nanopillars (SMGAPs) is described. The electrochemical reduction on the pre-distributed 40 nm gold nanoparticle seeds (sGNP) acted as scaffolds for silver ion growth, and a nanopillar-shaped silver structure was successfully grown on the gold substrate surface. The synthesized structure showed uniform and remarkably increased signal enhancement for malachite green isothiocyanate. Based on this consistency, two circulating miRNA markers for breast cancer (miR-21 and miR-155) were used as the SERS diagnostic target. The limit of detection (LOD) of each labeled target was 451 zmol and 1.65 amol respectively. Moreover, miRNAs in four types of cancer cell extracts (HCC1143, HCC1954, MDA-MB-231, MCF-7) were sorted by miR-21 and miR-155 copies. Finally, quantitative analysis of miRNA in urine was successful compared to that in the healthy group.
Full Text
https://www.sciencedirect.com/science/article/pii/S095656632200183X?via%3Dihub
DOI
10.1016/j.bios.2022.114143
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Son, Hye Yeong(손혜영) ORCID logo https://orcid.org/0000-0001-5977-6784
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/189268
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