Protein kinase CK2-dependent aerobic glycolysis-induced lactate dehydrogenase A enhances the migration and invasion of cancer cells

Abstract

We investigated the intracellular metabolic fluxes of protein kinase CK2-activating (C alpha OE) cells and role of lactate dehydrogenase A (LDHA) as a contributor of tumorigenesis after reprogrammed glucose metabolism. Facilitated aerobic glycolysis was confirmed via isotope tracer analysis, in which C-13(6)-Glc or C-13(5)-Gln was added to the media, following which metabolites converted from C alpha OE cells were identified. We found a greater decrease in cell survival, colony-forming ability, migration, and C alpha OE cell invasion under glucose (Glc)-depletion conditions than under glutamine (Gln)-depletion conditions. Cancer cell migration and invasion increased due to LDHA elevation of the altered metabolic axis driven by activated CK2. FX11 treatment and LDHA knockdown suppressed migration and invasion through ROS generation, but this was partially reversed by the antioxidant N-acetylcysteine (NAC). Moreover, LDHA inhibition decreased tumor growth in a mouse xenograft model transplanted with C alpha OE cells. Finally, we concluded that LDHA is an excellent metabolic target for tumor therapy, based on CK2 alpha derived aerobic glycolysis.