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NeuroTrace 500/525 identifies human induced pluripotent stem cell-derived brain pericyte-like cells

Authors
 Seo Young Kim  ;  Jihye Choi  ;  Junhee Roh  ;  Chul Hoon Kim 
Citation
 MOLECULAR BRAIN, Vol.15(1) : 11, 2022-01 
Journal Title
MOLECULAR BRAIN
Issue Date
2022-01
MeSH
Animals ; Blood-Brain Barrier ; Brain / blood supply ; Humans ; Induced Pluripotent Stem Cells* ; Mice ; Myocytes, Smooth Muscle ; Pericytes* / physiology
Keywords
NeuroTrace 500/525 ; Pericytes ; hiPSCs
Abstract
In the CNS, pericytes are important for maintaining the blood-brain barrier (BBB) and for controlling blood flow. Recently, several methods were suggested for the differentiation of human pluripotent stem cells (hPSCs) into brain mural cells, specifically pericytes or vascular smooth muscle cells (vSMCs). Unfortunately, identifying the pericytes from among such hPSC-derived mural cells has been challenging. This is due both to the lack of pericyte-specific markers and to the loss of defining anatomical information inherent to culture conditions. We therefore asked whether NeuroTrace 500/525, a newly developed dye that shows cell-specific uptake into pericytes in the mouse brain, can help identify human induced pluripotent stem cell (hiPSC)-derived brain pericyte-like cells. First, we found that NeuroTrace 500/525 specifically stains primary cultured human brain pericytes, confirming its specificity in vitro. Second, we found that NeuroTrace 500/525 specifically labels hiPSC-derived pericyte-like cells, but not endothelial cells or vSMCs derived from the same hiPSCs. Last, we found that neuroectoderm-derived vSMCs, which have pericyte-like features, also take up NeuroTrace 500/525. These data indicate NeuroTrace 500/525 is useful for identifying pericyte-like cells among hiPSC-derived brain mural cells.
Files in This Item:
T202201897.pdf Download
DOI
10.1186/s13041-021-00893-5
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chul Hoon(김철훈) ORCID logo https://orcid.org/0000-0002-7360-429X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/188689
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