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Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 directly binds and stabilizes Nrf2 in breast cancer

 Soma Saeidi  ;  Su-Jung Kim  ;  Yanymee N Guillen-Quispe  ;  Achanta Sri Venkata Jagadeesh  ;  Hyeong-Jun Han  ;  Seung Hyeon Kim  ;  Xiancai Zhong  ;  Juan-Yu Piao  ;  Seong-Jin Kim  ;  Joon Jeong  ;  Yun Jin Shin  ;  Yoon Jin Cha  ;  Han-Byoel Lee  ;  Wonshik Han  ;  Sang-Hyun Min  ;  Wang Tian  ;  Hiroshi Kitamura  ;  Young-Joon Surh 
 FASEB JOURNAL, Vol.36(1) : e22068, 2022-01 
Journal Title
Issue Date
Animals ; Breast Neoplasms / genetics ; Breast Neoplasms / metabolism* ; Female ; HEK293 Cells ; Humans ; MCF-7 Cells ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; NIMA-Interacting Peptidylprolyl Isomerase / genetics ; NIMA-Interacting Peptidylprolyl Isomerase / metabolism* ; Neoplasm Proteins / genetics ; Neoplasm Proteins / metabolism* ; Protein Binding ; Protein Stability ; Proteolysis ; Ubiquitination
Keap1 ; Nrf2 ; Pin1 ; breast cancer ; protein-protein interaction
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) has been frequently overexpressed in many types of malignancy, suggesting its oncogenic function. It recognizes phosphorylated serine or threonine (pSer/Thr) of a target protein and isomerizes the adjacent proline (Pro) residue, thereby altering folding, subcellular localization, stability, and function of target proteins. The oncogenic transcription factor, Nrf2 harbors the pSer/Thr-Pro motif. This prompted us to investigate whether Pin1 could bind to Nrf2 and influence its stability and function in the context of implications for breast cancer development and progression. The correlation between Pin1 and Nrf2 in the triple-negative breast cancer cells was validated by RNASeq analysis as well as immunofluorescence staining. Interaction between Pin1 and Nrf2 was assessed by co-immunoprecipitation and an in situ proximity ligation assay. We found that mRNA and protein levels of Pin1 were highly increased in the tumor tissues of triple-negative breast cancer patients and the human breast cancer cell line. Genetic or pharmacologic inhibition of Pin1 enhanced the ubiquitination and degradation of Nrf2. In contrast, the overexpression of Pin1 resulted in the accumulation of Nrf2 in the nucleus, without affecting its transcription. Notably, the phosphorylation of Nrf2 at serine 215, 408, and 577 is essential for its interaction with Pin1. We also identified phosphorylated Ser104 and Thr277 residues in Keap1, a negative regulator of Nrf2, for Pin1 binding. Pin1 plays a role in breast cancer progression through stabilization and constitutive activation of Nrf2 by competing with Keap1 for Nrf2 binding.
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1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Jeong, Joon(정준) ORCID logo https://orcid.org/0000-0003-0397-0005
Cha, Yoon Jin(차윤진) ORCID logo https://orcid.org/0000-0002-5967-4064
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