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Fine-tuning of epithelial taste bud organoid to promote functional recapitulation of taste reactivity

Authors
 Anish Ashok Adpaikar  ;  Sushan Zhang  ;  Hyun-Yi Kim  ;  Ki Woo Kim  ;  Seok Jun Moon  ;  Jong-Min Lee  ;  Han-Sung Jung 
Citation
 CELLULAR AND MOLECULAR LIFE SCIENCES, Vol.79(4) : 211, 2022-03 
Journal Title
CELLULAR AND MOLECULAR LIFE SCIENCES
ISSN
 1420-682X 
Issue Date
2022-03
MeSH
Animals ; Epithelium / physiology ; Mice ; Organoids ; Taste / physiology ; Taste Buds* / physiology ; Tongue / innervation
Keywords
Apicobasal polarity ; Calcium imaging ; Genetic alteration ; Single-cell RNA sequencing ; Suspension-culture ; Taste bud organoids ; Taste receptor cells ; Transplantation
Abstract
Taste stem/progenitor cells from posterior mouse tongues have been used to generate taste bud organoids. However, the inaccessible location of taste receptor cells is observed in conventional organoids. In this study, we established a suspension-culture method to fine-tune taste bud organoids by apicobasal polarity alteration to form the accessible localization of taste receptor cells. Compared to conventional Matrigel-embedded organoids, suspension-cultured organoids showed comparable differentiation and renewal rates to those of taste buds in vivo and exhibited functional taste receptor cells and cycling progenitor cells. Accessible taste receptor cells enabled the direct application of calcium imaging to evaluate the taste response. Moreover, suspension-cultured organoids can be genetically altered. Suspension-cultured taste bud organoids harmoniously integrated with the recipient lingual epithelium, maintaining the taste receptor cells and gustatory innervation capacity. We propose that suspension-cultured organoids may provide an efficient model for taste research, including taste bud development, regeneration, and transplantation.
Files in This Item:
T202201014.pdf Download
DOI
10.1007/s00018-022-04242-0
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Kim, Ki Woo(김기우) ORCID logo https://orcid.org/0000-0002-7790-1515
Moon, Seok Jun(문석준) ORCID logo https://orcid.org/0000-0001-7282-2888
Lee, Jong Min(이종민) ORCID logo https://orcid.org/0000-0002-9466-7644
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/188385
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