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A Bioprinting Process Supplemented with In Situ Electrical Stimulation Directly Induces Significant Myotube Formation and Myogenesis

Authors
 WonJin Kim  ;  Hyeongjin Lee  ;  Chang Kyu Lee  ;  Jae Won Kyung  ;  Seong Bae An  ;  In-Bo Han  ;  Geun Hyung Kim 
Citation
 ADVANCED FUNCTIONAL MATERIALS, Vol.31(51) : 2105170, 2021-12 
Journal Title
ADVANCED FUNCTIONAL MATERIALS
ISSN
 1616-301X 
Issue Date
2021-12
Abstract
Electric field stimulation has supported biophysical and biological cues for tissue regeneration approaches to affect cell morphology, alignment, and even cellular phenotypes types. Here, an innovative bioprinting approach supported by in situ electrical stiumlation (E-printing) is used to fabricate a bioengineered skeletal muscle construct composed of human adipose stem cells and methacrylated decellularized extracellular matrix (dECM-Ma) derived from porcine muscle. To obtain highly ordered myofiber-like structures, various parameters of the printing process are optimized. The E-printed structure exhibits higher cell viability and fully aligned cytoskeleton than the conventionally printed cell-bearing structures, due to activation of voltage-gated ion channels that affect various signaling pathways. When using the E-printed structure, expression of myogenesis-related genes is upregulated by 1.9–2.5-fold higher than when using a dECM-Ma structure produced without electrical stimulation. Furthermore, when implanted into a rat model of volumetric muscle loss, the structure yields outstanding myogenesis relative to the conventionally bioprinted structure.
Full Text
https://onlinelibrary.wiley.com/doi/full/10.1002/adfm.202105170
DOI
10.1002/adfm.202105170
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Chang Kyu(이창규)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/188164
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