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Impaired antibacterial response of liver sinusoidal Vγ9 + Vδ2 + T cells in patients with chronic liver disease

DC Field Value Language
dc.contributor.author김명수-
dc.contributor.author김순일-
dc.contributor.author김종훈-
dc.contributor.author박준용-
dc.contributor.author이재근-
dc.contributor.author주동진-
dc.date.accessioned2022-03-11T06:22:20Z-
dc.date.available2022-03-11T06:22:20Z-
dc.date.issued2022-03-
dc.identifier.issn0017-5749-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/188056-
dc.description.abstractObjective: The liver acts as a frontline barrier against diverse gut-derived pathogens, and the sinusoid is the primary site of liver immune surveillance. However, little is known about liver sinusoidal immune cells in the context of chronic liver disease (CLD). Here, we investigated the antibacterial capacity of liver sinusoidal γδ T cells in patients with various CLDs. Design: We analysed the frequency, phenotype and functions of human liver sinusoidal γδ T cells from healthy donors and recipients with CLD, including HBV-related CLD (liver cirrhosis (LC) and/or hepatocellular carcinoma (HCC)), alcoholic LC and LC or HCC of other aetiologies, by flow cytometry and RNA-sequencing using liver perfusates obtained during living donor liver transplantation. We also measured the plasma levels of D-lactate and bacterial endotoxin to evaluate bacterial translocation. Results: The frequency of liver sinusoidal Vγ9+Vδ2+ T cells was reduced in patients with CLD. Immunophenotypic and transcriptomic analyses revealed that liver sinusoidal Vγ9+Vδ2+ T cells from patients with CLD were persistently activated and pro-apoptotic. In addition, liver sinusoidal Vγ9+Vδ2+ T cells from patients with CLD showed significantly decreased interferon (IFN)-γ production following stimulation with bacterial metabolites and Escherichia coli. The antibacterial IFN-γ response of liver sinusoidal Vγ9+Vδ2+ T cells significantly correlated with liver function, and inversely correlated with the plasma level of D-lactate in patients with CLD. Repetitive in vitro stimulation with E. coli induced activation, apoptosis and functional impairment of liver sinusoidal Vγ9+Vδ2+ T cells. Conclusion: Liver sinusoidal Vγ9+Vδ2+ T cells are functionally impaired in patients with CLD. Bacterial translocation and decreasing liver functions are associated with functional impairment of liver sinusoidal Vγ9+Vδ2+ T cells.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherBritish Medical Assn.-
dc.relation.isPartOfGUT-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleImpaired antibacterial response of liver sinusoidal Vγ9 + Vδ2 + T cells in patients with chronic liver disease-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Surgery (외과학교실)-
dc.contributor.googleauthorMin-Seok Rha-
dc.contributor.googleauthorJi Won Han-
dc.contributor.googleauthorJune-Young Koh-
dc.contributor.googleauthorHa Seok Lee-
dc.contributor.googleauthorJong Hoon Kim-
dc.contributor.googleauthorKyungjoo Cho-
dc.contributor.googleauthorSoon Il Kim-
dc.contributor.googleauthorMyoung Soo Kim-
dc.contributor.googleauthorJae Geun Lee-
dc.contributor.googleauthorSu-Hyung Park-
dc.contributor.googleauthorDong Jin Joo-
dc.contributor.googleauthorJun Yong Park-
dc.contributor.googleauthorEui-Cheol Shin-
dc.identifier.doi10.1136/gutjnl-2020-322182-
dc.contributor.localIdA00424-
dc.contributor.localIdA00649-
dc.contributor.localIdA05233-
dc.contributor.localIdA01675-
dc.contributor.localIdA02137-
dc.contributor.localIdA03068-
dc.contributor.localIdA03948-
dc.relation.journalcodeJ00953-
dc.identifier.eissn1468-3288-
dc.identifier.pmid33472894-
dc.identifier.urlhttps://gut.bmj.com/content/71/3/605-
dc.subject.keywordbacterial translocation-
dc.subject.keywordchronic liver disease-
dc.subject.keywordgamma-delta cells-
dc.subject.keywordliver immunology-
dc.contributor.alternativeNameKim, Myoung Soo-
dc.contributor.affiliatedAuthor김명수-
dc.contributor.affiliatedAuthor김순일-
dc.contributor.affiliatedAuthor김종훈-
dc.contributor.affiliatedAuthor박준용-
dc.contributor.affiliatedAuthor이재근-
dc.contributor.affiliatedAuthor주동진-
dc.citation.volume71-
dc.citation.number3-
dc.citation.startPage605-
dc.citation.endPage615-
dc.identifier.bibliographicCitationGUT, Vol.71(3) : 605-615, 2022-03-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

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