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SEPHS1 is dispensable for pluripotency maintenance but indispensable for cardiac differentiation in mouse embryonic stem cells

Authors
 Lu Qiao  ;  So Hee Dho  ;  Ji Young Kim  ;  Lark Kyun Kim 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.590 : 125-131, 2022-01 
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN
 0006-291X 
Issue Date
2022-01
MeSH
Animals ; Cell Differentiation* / genetics ; Embryoid Bodies / cytology ; Gastrulation / genetics ; Gene Expression Regulation, Developmental ; Germ Layers / cytology ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mouse Embryonic Stem Cells / cytology* ; Mouse Embryonic Stem Cells / metabolism* ; Myocardium / cytology* ; Phosphotransferases / deficiency ; Phosphotransferases / metabolism* ; Transcription, Genetic
Keywords
Cardiac development ; Embryoid bodies ; Embryonic stem cells ; Gastruloids ; In vitro differentiation ; SEPHS1
Abstract
Embryonic stem cells (ESCs) are derived from the inner cell mass of developing blastocysts, which have self-renewal ability and have the potential to develop or reconstitute into all embryonic lineages. Selenophosphate synthetase 1 (SEPHS1) is an essential protein in mouse early embryo development. However, the role of SEPHS1 in mouse ESCs remains to be elucidated. In this study, we generated Sephs1 KO ESCs and found that deficiency of SEPSH1 has little effect on pluripotency maintenance and proliferation. Notably, SEPHS1 deficiency impaired differentiation into three germ layers and gastruloid aggregation in vitro. RNA-seq analysis showed SEPHS1 is involved in cardiogenesis, verified by no beating signal in Sephs1 KO embryoid body at d10 and low expression of cardiac-related and contraction markers. Taken together, our results suggest that SPEHS1 is dispensable in ESC self-renewal, but indispensable in subsequent germ layer differentiation especially for functional cardiac lineage.
Full Text
https://www.sciencedirect.com/science/article/pii/S0006291X21017423
DOI
10.1016/j.bbrc.2021.12.091
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Kim, Lark Kyun(김락균) ORCID logo https://orcid.org/0000-0001-5983-4470
Dho, So Hee(도소희) ORCID logo https://orcid.org/0000-0001-7343-713X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/187961
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