38 74

Cited 0 times in

CTCF-mediated chromatin looping provides a topological framework for the formation of phase-separated transcriptional condensates

DC Field Value Language
dc.contributor.author김형표-
dc.date.accessioned2022-03-11T05:55:24Z-
dc.date.available2022-03-11T05:55:24Z-
dc.date.issued2022-01-
dc.identifier.issn0305-1048-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/187866-
dc.description.abstractCTCF is crucial to the organization of mammalian genomes into loop structures. According to recent studies, the transcription apparatus is compartmentalized and concentrated at super-enhancers to form phase-separated condensates and drive the expression of cell-identity genes. However, it remains unclear whether and how transcriptional condensates are coupled to higher-order chromatin organization. Here, we show that CTCF is essential for RNA polymerase II (Pol II)-mediated chromatin interactions, which occur as hyperconnected spatial clusters at super-enhancers. We also demonstrate that CTCF clustering, unlike Pol II clustering, is independent of liquid-liquid phase-separation and resistant to perturbation of transcription. Interestingly, clusters of Pol II, BRD4, and MED1 were found to dissolve upon CTCF depletion, but were reinstated upon restoration of CTCF, suggesting a potent instructive function for CTCF in the formation of transcriptional condensates. Overall, we provide evidence suggesting that CTCF-mediated chromatin looping acts as an architectural prerequisite for the assembly of phase-separated transcriptional condensates.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherOxford University Press-
dc.relation.isPartOfNUCLEIC ACIDS RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHCCCTC-Binding Factor / metabolism*-
dc.subject.MESHCell Cycle Proteins / genetics-
dc.subject.MESHCell Cycle Proteins / metabolism-
dc.subject.MESHChromatin / chemistry-
dc.subject.MESHChromatin / genetics-
dc.subject.MESHChromatin / metabolism-
dc.subject.MESHChromatin Assembly and Disassembly*-
dc.subject.MESHEpigenesis, Genetic-
dc.subject.MESHHCT116 Cells-
dc.subject.MESHHumans-
dc.subject.MESHMediator Complex Subunit 1 / genetics-
dc.subject.MESHMediator Complex Subunit 1 / metabolism-
dc.subject.MESHRNA Polymerase II / metabolism-
dc.subject.MESHTranscription Factors / genetics-
dc.subject.MESHTranscription Factors / metabolism-
dc.titleCTCF-mediated chromatin looping provides a topological framework for the formation of phase-separated transcriptional condensates-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학교실)-
dc.contributor.googleauthorRyanggeun Lee-
dc.contributor.googleauthorMoo-Koo Kang-
dc.contributor.googleauthorYong-Jin Kim-
dc.contributor.googleauthorBobae Yang-
dc.contributor.googleauthorHwanyong Shim-
dc.contributor.googleauthorSugyung Kim-
dc.contributor.googleauthorKyungwoo Kim-
dc.contributor.googleauthorChul Min Yang-
dc.contributor.googleauthorByeong-Gyu Min-
dc.contributor.googleauthorWoong-Jae Jung-
dc.contributor.googleauthorEun-Chong Lee-
dc.contributor.googleauthorJung-Sik Joo-
dc.contributor.googleauthorGunhee Park-
dc.contributor.googleauthorWon-Ki Cho-
dc.contributor.googleauthorHyoung-Pyo Kim-
dc.identifier.doi10.1093/nar/gkab1242-
dc.contributor.localIdA01163-
dc.relation.journalcodeJ02387-
dc.identifier.eissn1362-4962-
dc.identifier.pmid34931241-
dc.contributor.alternativeNameKim, Hyoung Pyo-
dc.contributor.affiliatedAuthor김형표-
dc.citation.volume50-
dc.citation.number1-
dc.citation.startPage207-
dc.citation.endPage226-
dc.identifier.bibliographicCitationNUCLEIC ACIDS RESEARCH, Vol.50(1) : 207-226, 2022-01-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.