Cited 2 times in
Nano-Sized Extracellular Matrix Particles Lead to Therapeutic Improvement for Cutaneous Wound and Hindlimb Ischemia
DC Field | Value | Language |
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dc.contributor.author | 최동훈 | - |
dc.date.accessioned | 2022-02-23T01:07:03Z | - |
dc.date.available | 2022-02-23T01:07:03Z | - |
dc.date.issued | 2021-12 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/187545 | - |
dc.description.abstract | Cell-derived matrix (CDM) has proven its therapeutic potential and been utilized as a promising resource in tissue regeneration. In this study, we prepared a human fibroblast-derived matrix (FDM) by decellularization of in vitro cultured cells and transformed the FDM into a nano-sized suspended formulation (sFDM) using ultrasonication. The sFDM was then homogeneously mixed with Pluronic F127 and hyaluronic acid (HA), to effectively administer sFDM into target sites. Both sFDM and sFDM containing hydrogel (PH/sFDM) were characterized via immunofluorescence, sol-gel transition, rheological analysis, and biochemical factors array. We found that PH/sFDM hydrogel has biocompatible, mechanically stable, injectable properties and can be easily administered into the external and internal target regions. sFDM itself holds diverse bioactive molecules. Interestingly, sFDM-containing serum-free media helped maintain the metabolic activity of endothelial cells significantly better than those in serum-free condition. PH/sFDM also promoted vascular endothelial growth factor (VEGF) secretion from monocytes in vitro. Moreover, when we evaluated therapeutic effects of PH/sFDM via the murine full-thickness skin wound model, regenerative potential of PH/sFDM was supported by epidermal thickness, significantly more neovessel formation, and enhanced mature collagen deposition. The hindlimb ischemia model also found some therapeutic improvements, as assessed by accelerated blood reperfusion and substantially diminished necrosis and fibrosis in the gastrocnemius and tibialis muscles. Together, based on sFDM holding a strong therapeutic potential, our engineered hydrogel (PH/sFDM) should be a promising candidate in tissue engineering and regenerative medicine. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.publisher | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Extracellular Matrix / chemistry* | - |
dc.subject.MESH | Fibroblasts / chemistry* | - |
dc.subject.MESH | Hindlimb / drug effects | - |
dc.subject.MESH | Hindlimb / injuries* | - |
dc.subject.MESH | Human Umbilical Vein Endothelial Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hyaluronic Acid / chemistry | - |
dc.subject.MESH | Hyaluronic Acid / pharmacology* | - |
dc.subject.MESH | Hydrogels | - |
dc.subject.MESH | Ischemia / drug therapy* | - |
dc.subject.MESH | Ischemia / etiology | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Particle Size | - |
dc.subject.MESH | Poloxamer / chemistry | - |
dc.subject.MESH | Regenerative Medicine | - |
dc.subject.MESH | Rheology | - |
dc.subject.MESH | THP-1 Cells | - |
dc.subject.MESH | Vascular Endothelial Growth Factor A / metabolism | - |
dc.subject.MESH | Wound Healing / drug effects* | - |
dc.title | Nano-Sized Extracellular Matrix Particles Lead to Therapeutic Improvement for Cutaneous Wound and Hindlimb Ischemia | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Sang Su Ha | - |
dc.contributor.googleauthor | Jung-Hyun Kim | - |
dc.contributor.googleauthor | Cininta Savitri | - |
dc.contributor.googleauthor | Donghoon Choi | - |
dc.contributor.googleauthor | Kwideok Park | - |
dc.identifier.doi | 10.3390/ijms222413265 | - |
dc.contributor.localId | A04053 | - |
dc.relation.journalcode | J01133 | - |
dc.identifier.eissn | 1422-0067 | - |
dc.identifier.pmid | 34948061 | - |
dc.subject.keyword | Pluronic F127 | - |
dc.subject.keyword | decellularization | - |
dc.subject.keyword | fibroblast-derived matrix (FDM) | - |
dc.subject.keyword | hindlimb ischemia | - |
dc.subject.keyword | hyaluronic acid | - |
dc.subject.keyword | suspended FDM | - |
dc.subject.keyword | wound healing | - |
dc.contributor.alternativeName | Choi, Dong Hoon | - |
dc.contributor.affiliatedAuthor | 최동훈 | - |
dc.citation.volume | 22 | - |
dc.citation.number | 24 | - |
dc.citation.startPage | 13265 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.22(24) : 13265, 2021-12 | - |
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