Cited 26 times in
Multi-miRNA panel of tumor-derived extracellular vesicles as promising diagnostic biomarkers of early-stage breast cancer
DC Field | Value | Language |
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dc.contributor.author | 김승일 | - |
dc.contributor.author | 김지예 | - |
dc.contributor.author | 김민우 | - |
dc.date.accessioned | 2022-02-23T01:04:11Z | - |
dc.date.available | 2022-02-23T01:04:11Z | - |
dc.date.issued | 2021-12 | - |
dc.identifier.issn | 1347-9032 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/187523 | - |
dc.description.abstract | Extracellular vesicles (EV) have been emerging as potential biomarkers for disease monitoring. In particular, tumor-derived EV (TDE) are known to carry oncogenic miRNA, so they can be used for diagnosis of early cancer by analyzing the expression levels of EV-miRNA circulating in the blood. Here, using our novel microfluidic device, we rapidly and selectively isolate cancerous EV expressing breast cancer-derived surface markers CD49f and EpCAM within 2 minutes. Based on seven candidates of miRNA nominated from The Cancer Genome Atlas (TCGA) database, the expression levels of miRNA in TDE were validated in a total of 82 individuals, including 62 breast cancer patients and 20 healthy controls. Among seven candidates, four miRNAs (miR-9, miR-16, miR-21, and miR-429) from the EV were highly elevated in early-stage breast cancer patients compared with healthy donors. The combination of significant miRNAs from specific EV has high sensitivities of 0.90, 0.86, 0.88, and 0.84 of the area under the receiver operating characteristic curve (AUC) in each subtype (luminal A, luminal B, HER-2, and triple-negative) of early-stage breast cancer. Our results suggest that the combination of four miRNA signatures of specific EV could serve as a sensitive and specific biomarker and enable early diagnosis of breast cancer using liquid biopsy. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Wiley Publishing on behalf of the Japanese Cancer Association | - |
dc.relation.isPartOf | CANCER SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Biomarkers, Tumor / genetics | - |
dc.subject.MESH | Breast Neoplasms / diagnosis* | - |
dc.subject.MESH | Breast Neoplasms / genetics | - |
dc.subject.MESH | Breast Neoplasms / pathology | - |
dc.subject.MESH | Case-Control Studies | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Databases, Genetic | - |
dc.subject.MESH | Early Detection of Cancer | - |
dc.subject.MESH | Epithelial Cell Adhesion Molecule / metabolism | - |
dc.subject.MESH | Extracellular Vesicles / genetics* | - |
dc.subject.MESH | Extracellular Vesicles / metabolism | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gene Expression Profiling | - |
dc.subject.MESH | Gene Expression Regulation, Neoplastic | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Integrin alpha6 / metabolism | - |
dc.subject.MESH | MCF-7 Cells | - |
dc.subject.MESH | MicroRNAs / genetics* | - |
dc.subject.MESH | Microfluidic Analytical Techniques / instrumentation | - |
dc.subject.MESH | Neoplasm Staging | - |
dc.subject.MESH | Up-Regulation* | - |
dc.title | Multi-miRNA panel of tumor-derived extracellular vesicles as promising diagnostic biomarkers of early-stage breast cancer | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학교실) | - |
dc.contributor.googleauthor | Min Woo Kim | - |
dc.contributor.googleauthor | Sunyoung Park | - |
dc.contributor.googleauthor | Hyojung Lee | - |
dc.contributor.googleauthor | Hogyeong Gwak | - |
dc.contributor.googleauthor | Kyung-A Hyun | - |
dc.contributor.googleauthor | Jee Ye Kim | - |
dc.contributor.googleauthor | Hyo-Il Jung | - |
dc.contributor.googleauthor | Seung Il Kim | - |
dc.identifier.doi | 10.1111/cas.15155 | - |
dc.contributor.localId | A00658 | - |
dc.contributor.localId | A00984 | - |
dc.relation.journalcode | J00454 | - |
dc.identifier.eissn | 1349-7006 | - |
dc.identifier.pmid | 34626053 | - |
dc.subject.keyword | biomarker | - |
dc.subject.keyword | early-stage breast cancer | - |
dc.subject.keyword | extracellular vesicles | - |
dc.subject.keyword | liquid biopsy | - |
dc.subject.keyword | microRNA | - |
dc.contributor.alternativeName | Kim, Seung Il | - |
dc.contributor.affiliatedAuthor | 김승일 | - |
dc.contributor.affiliatedAuthor | 김지예 | - |
dc.citation.volume | 112 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 5078 | - |
dc.citation.endPage | 5087 | - |
dc.identifier.bibliographicCitation | CANCER SCIENCE, Vol.112(12) : 5078-5087, 2021-12 | - |
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