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Hypoxia Regulates the Extracellular Matrix via Mitogen-Activated Protein Kinases Pathway in Cells Retrieved from the Human Intervertebral Disc

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dc.contributor.author문성환-
dc.contributor.author양재호-
dc.contributor.author이병호-
dc.contributor.author이환모-
dc.date.accessioned2022-02-23T01:02:27Z-
dc.date.available2022-02-23T01:02:27Z-
dc.date.issued2021-08-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/187507-
dc.description.abstractPurpose: The present study aimed to identify the physiological characteristics of cells by investigating the change in gene expression and protein levels during extracellular matrix (ECM) synthesis in the intervertebral disc (IVD) under hypoxic conditions. Materials and methods: To test the effect of oxygen on cell growth and ECM synthesis of chondrocyte-like cells, the cells from IVD were separated and cultured in two hypoxia-mimicking systems: chemical hypoxic conditions using deferoxamine (DFO), and physiological hypoxic conditions using a hypoxic chamber for 7 days. Chondrocyte like cells cultured without DFO and under the normal oxygen concentration (21% O₂ and 5% CO₂, 37°C) served as the controls. Results: Chondrocyte-like cells cultured in the presence of 6% oxygen demonstrated a 100% increase in cellular proliferation compared to the control. The cells treated with chemical hypoxic conditions demonstrated a dose-dependent increase in the mRNA expression of glucose transporter-1, GAPDH, aggrecan, and type II collagen on Day 1. When treated with 100 µM DFO, the cells showed a 50% increase in the levels of proteoglycan protein on Day 7. The cells treated with chemical hypoxic condition demonstrated increase in sulfated glycosaminoglycan (GAG) protein levels on Day 7. Moreover, the cells cultured in the presence of 6% oxygen showed a 120% increase in sulfated GAG levels on Day 7. Conclusion: The oxygen concentration had an important role in the viability, proliferation, and maturation of chondrocyte-like cells in IVD. In addition, chondrocyte-like cells are sensitive to the concentration of oxygen.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherYonsei University-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAggrecans / genetics-
dc.subject.MESHCells, Cultured-
dc.subject.MESHExtracellular Matrix-
dc.subject.MESHHumans-
dc.subject.MESHHypoxia-
dc.subject.MESHIntervertebral Disc*-
dc.subject.MESHMitogen-Activated Protein Kinases*-
dc.titleHypoxia Regulates the Extracellular Matrix via Mitogen-Activated Protein Kinases Pathway in Cells Retrieved from the Human Intervertebral Disc-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Orthopedic Surgery (정형외과학교실)-
dc.contributor.googleauthorYoung Mi Kang-
dc.contributor.googleauthorEun Jung Shin-
dc.contributor.googleauthorByung Ho Lee-
dc.contributor.googleauthorJae Ho Yang-
dc.contributor.googleauthorHwan Mo Lee-
dc.contributor.googleauthorSeong Hwan Moon-
dc.identifier.doi10.3349/ymj.2021.62.8.734-
dc.contributor.localIdA01365-
dc.contributor.localIdA02317-
dc.contributor.localIdA02801-
dc.contributor.localIdA03333-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid34296551-
dc.subject.keywordIntervertebral disc-
dc.subject.keywordchondrocyte-like cell-
dc.subject.keywordextracellular matrix-
dc.subject.keywordlow oxygen-
dc.subject.keywordproteoglycan-
dc.contributor.alternativeNameMoon, Seong Hwan-
dc.contributor.affiliatedAuthor문성환-
dc.contributor.affiliatedAuthor양재호-
dc.contributor.affiliatedAuthor이병호-
dc.contributor.affiliatedAuthor이환모-
dc.citation.volume62-
dc.citation.number8-
dc.citation.startPage734-
dc.citation.endPage742-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.62(8) : 734-742, 2021-08-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers

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