Cited 16 times in
Isolation of Antibodies Against the Spike Protein of SARS-CoV from Pig Serum for Competitive Immunoassay
DC Field | Value | Language |
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dc.contributor.author | 김현옥 | - |
dc.date.accessioned | 2022-02-23T00:56:41Z | - |
dc.date.available | 2022-02-23T00:56:41Z | - |
dc.date.issued | 2021-12 | - |
dc.identifier.issn | 1976-0280 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/187453 | - |
dc.description.abstract | Several endemic corona viruses (eCoVs) have been reported to be the most common etiologic agents for the seasonal common cold and also cause pneumonia. These eCoVs share extensive sequence homology with SARS-CoV-2, and immune responses to eCoVs can cross-react with SARS-CoV-2 antigens. Based on such cross-reactivity of antigens among eCoVs, the IgG antibodies against the spike protein (SP) of severe acute respiratory syndrome coronavirus (SARS-CoV) were isolated from pig serum using magnetic beads immobilized with SARS-CoV SP and a protein-A column. The selectivity of the isolated antibodies was tested using different types of antigens, such as SARS-CoV-2 nucleoprotein (NP), influenza A virus (Beijing type), influenza B virus (Tokio and Florida types), human hepatitis B virus surface antigen (HBsAg), and bovine serum albumin (BSA). From the selectivity test, the anti-SP antibodies isolated from pig serum had sufficient selectivity to other kinds of viral antigens, and the apparent binding constant of the isolated antibodies was approximately 1.5 × 10-8 M from the surface plasmon resonance (SPR) measurements. Finally, the isolated anti-SP antibodies were applied to the immunoassay of SP using competitive immunoassay configuration. The feasibility of the detection as well as the quantitative analysis of the SARS-CoV viral culture fluid was determined using four viral culture samples, namely, SARS-CoV, SARS-CoV-2, MERS-CoV, and CoV-229E. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Korean Biochip Society | - |
dc.relation.isPartOf | BIOCHIP JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Isolation of Antibodies Against the Spike Protein of SARS-CoV from Pig Serum for Competitive Immunoassay | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Jaeyong Jung | - |
dc.contributor.googleauthor | Ji-Hong Bong | - |
dc.contributor.googleauthor | Tae-Hun Kim | - |
dc.contributor.googleauthor | Jeong Soo Sung | - |
dc.contributor.googleauthor | Changkyu Lee | - |
dc.contributor.googleauthor | Min-Jung Kang | - |
dc.contributor.googleauthor | Hyun Ok Kim | - |
dc.contributor.googleauthor | Hyun-Jin Shin | - |
dc.contributor.googleauthor | Jae-Chul Pyun | - |
dc.identifier.doi | 10.1007/s13206-021-00033-0 | - |
dc.contributor.localId | A01122 | - |
dc.relation.journalcode | J00292 | - |
dc.identifier.eissn | 2092-7843 | - |
dc.identifier.pmid | 34466204 | - |
dc.subject.keyword | Antibody isolation | - |
dc.subject.keyword | Competitive immunoassay | - |
dc.subject.keyword | Pig serum | - |
dc.subject.keyword | SARS-CoV spike protein | - |
dc.contributor.alternativeName | Kim, Hyun Ok | - |
dc.contributor.affiliatedAuthor | 김현옥 | - |
dc.citation.volume | 15 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 396 | - |
dc.citation.endPage | 405 | - |
dc.identifier.bibliographicCitation | BIOCHIP JOURNAL, Vol.15(4) : 396-405, 2021-12 | - |
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