Cited 82 times in
Clustered Regularly Interspaced Short Palindromic Repeats-Mediated Surface-Enhanced Raman Scattering Assay for Multidrug-Resistant Bacteria
DC Field | Value | Language |
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dc.contributor.author | 용동은 | - |
dc.date.accessioned | 2022-01-17T08:02:50Z | - |
dc.date.available | 2022-01-17T08:02:50Z | - |
dc.date.issued | 2020-11 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/187351 | - |
dc.description.abstract | Antimicrobial resistance and multidrug resistance are slower-moving pandemics than the fast-spreading coronavirus disease 2019; however, they have potential to cause a much greater threat to global health. Here, we report a clustered regularly interspaced short palindromic repeats (CRISPR)-mediated surface-enhanced Raman scattering (SERS) assay for multidrug-resistant (MDR) bacteria. This assay was developed via a synergistic combination of the specific gene-recognition ability of the CRISPR system, superb sensitivity of SERS, and simple separation property of magnetic nanoparticles. This assay detects three multidrug-resistant (MDR) bacteria, species Staphylococcus aureus, Acinetobacter baumannii, and Klebsiella pneumoniae, without purification or gene amplification steps. Furthermore, MDR A. baumannii-infected mice were successfully diagnosed using the assay. Finally, we demonstrate the on-site capture and detection of MDR bacteria through a combination of the three-dimensional nanopillar array swab and CRISPR-mediated SERS assay. This method may prove effective for the accurate diagnosis of MDR bacterial pathogens, thus preventing severe infection by ensuring appropriate antibiotic treatment. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | American Chemical Society | - |
dc.relation.isPartOf | ACS NANO | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Clustered Regularly Interspaced Short Palindromic Repeats-Mediated Surface-Enhanced Raman Scattering Assay for Multidrug-Resistant Bacteria | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Hongki Kim | - |
dc.contributor.googleauthor | Soohyun Lee | - |
dc.contributor.googleauthor | Hwi Won Seo | - |
dc.contributor.googleauthor | Byunghoon Kang | - |
dc.contributor.googleauthor | Jeong Moon | - |
dc.contributor.googleauthor | Kyoung G Lee | - |
dc.contributor.googleauthor | Dongeun Yong | - |
dc.contributor.googleauthor | Hyunju Kang | - |
dc.contributor.googleauthor | Juyeon Jung | - |
dc.contributor.googleauthor | Eun-Kyung Lim | - |
dc.contributor.googleauthor | Jinyoung Jeong | - |
dc.contributor.googleauthor | Hyun Gyu Park | - |
dc.contributor.googleauthor | Choong-Min Ryu | - |
dc.contributor.googleauthor | Taejoon Kang | - |
dc.identifier.doi | 10.1021/acsnano.0c07264 | - |
dc.contributor.localId | A02423 | - |
dc.contributor.localId | A04404 | - |
dc.relation.journalcode | J00005 | - |
dc.identifier.eissn | 1936-086X | - |
dc.identifier.pmid | 33216524 | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acsnano.0c07264 | - |
dc.subject.keyword | CRISPR/dCas9 | - |
dc.subject.keyword | antimicrobial-resistance | - |
dc.subject.keyword | bacteria | - |
dc.subject.keyword | nanoparticle | - |
dc.subject.keyword | surface-enhanced Raman scattering | - |
dc.contributor.alternativeName | Yong, Dong Eun | - |
dc.contributor.affiliatedAuthor | 용동은 | - |
dc.citation.volume | 14 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 17241 | - |
dc.citation.endPage | 17253 | - |
dc.identifier.bibliographicCitation | ACS NANO, Vol.14(12) : 17241-17253, 2020-11 | - |
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