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Sterilization of sealed PVDF pouches containing decellularized scaffold by electrical stimulation

Authors
 Mi Hee Lee  ;  HaKyeong Jeong  ;  Min-Ah Koo  ;  Gyeung Mi Seon  ;  Seung Hee Hong  ;  Ye Jin Park  ;  Jong-Chul Park 
Citation
 BIOTECHNOLOGY JOURNAL, Vol.16(11) : e2100156, 2021-08 
Journal Title
BIOTECHNOLOGY JOURNAL
ISSN
 1860-6768 
Issue Date
2021-08
Keywords
decellularized human dermal skin tissues ; electrical stimulation ; polyvinylidene fluoride pouch ; sterility assurance level ; tissue engineering products ; wet sterilization
Abstract
A terminal sterilization process for tissue engineering products, such as allografts and biomaterials is necessary to ensure complete removal of pathogenic microorganisms such as the bacteria, fungi, and viruses. However, it can be difficult to sterilize allografts and artificial tissue models packaged in wet conditions without deformation. In this study, we investigated the sterilization effects of electrical stimulation (ES) and assessed its suitability by evaluating sterility assurance levels in pouches at a constant current. Stability of polyvinylidene fluoride pouches was determined by a sterility test performed after exposure to five microorganisms (Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans) for 5 days; the sterility test was also performed with decellularized human dermal tissues inoculated with the five microorganisms. Sterilization using ES inactivated microorganisms both inside and outside of sealed pouches and caused no damage to the packaged tissue. Our results support the development of a novel system that involves ES sterilization for packaging of implantable biomaterials and human derived materials.
Full Text
https://onlinelibrary.wiley.com/doi/10.1002/biot.202100156
DOI
10.1002/biot.202100156
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Koo, Min-Ah(구민아) ORCID logo https://orcid.org/0000-0002-8671-1131
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Lee, Mi Hee(이미희) ORCID logo https://orcid.org/0000-0002-9630-7044
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/187202
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