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Naegleria fowleri Induces Jurkat T Cell Death via O-deGlcNAcylation

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dc.contributor.author신명헌-
dc.contributor.author이영아-
dc.date.accessioned2021-12-28T17:28:06Z-
dc.date.available2021-12-28T17:28:06Z-
dc.date.issued2021-10-
dc.identifier.issn0023-4001-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/187115-
dc.description.abstractThe pathogenic free-living amoeba Naegleria fowleri causes primary amoebic meningoencephalitis, a fatal infection, by penetrating the nasal mucosa and migrating to the brain via the olfactory nerves. N. fowleri can induce host cell death via lytic necrosis. Similar to phosphorylation, O-linked β-N-acetylglucosamine (O-GlcNAc) glycosylation (O-GlcNAcylation) is involved in various cell-signaling processes, including apoptosis and proliferation, with O-GlcNAc addition and removal regulated by O-GlcNAc transferase and O-GlcNAcase (OGA), respectively. However, the detailed mechanism of host cell death induced by N. fowleri is unknown. In this study, we investigated whether N. fowleri can induce the modulation of O-GlcNAcylated proteins during cell death in Jurkat T cells. Co-incubation with live N. fowleri trophozoites increased DNA fragmentation. In addition, incubation with N. fowleri induced a dramatic reduction in O-GlcNAcylated protein levels in 30 min. Moreover, pretreatment of Jurkat T cells with the OGA inhibitor PUGNAc prevented N. fowleri-induced O-deGlcNAcylation and DNA fragmentation. These results suggest that O-deGlcNAcylation is an important signaling process that occurs during Jurkat T cell death induced by N. fowleri.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish, Korean-
dc.publisherKorean Society for Parasitology-
dc.relation.isPartOfKOREAN JOURNAL OF PARASITOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAnimals-
dc.subject.MESHApoptosis-
dc.subject.MESHCell Death-
dc.subject.MESHHumans-
dc.subject.MESHJurkat Cells-
dc.subject.MESHNaegleria fowleri*-
dc.subject.MESHSignal Transduction-
dc.subject.MESHTrophozoites-
dc.titleNaegleria fowleri Induces Jurkat T Cell Death via O-deGlcNAcylation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학교실)-
dc.contributor.googleauthorYoung Ah Lee-
dc.contributor.googleauthorKyeong Ah Kim-
dc.contributor.googleauthorMyeong Heon Shin-
dc.identifier.doi10.3347/kjp.2021.59.5.501-
dc.contributor.localIdA02099-
dc.contributor.localIdA02959-
dc.relation.journalcodeJ01957-
dc.identifier.eissn1738-0006-
dc.identifier.pmid34724770-
dc.subject.keywordNaegleria fowleri-
dc.subject.keywordO-GlcNAcylation-
dc.subject.keywordhost cell death-
dc.contributor.alternativeNameShin, Myeong Heon-
dc.contributor.affiliatedAuthor신명헌-
dc.contributor.affiliatedAuthor이영아-
dc.citation.volume59-
dc.citation.number5-
dc.citation.startPage501-
dc.citation.endPage505-
dc.identifier.bibliographicCitationKOREAN JOURNAL OF PARASITOLOGY, Vol.59(5) : 501-505, 2021-10-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers

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