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Carbon-Ion Beam Irradiation and the miR-200c Mimic Effectively Eradicate Pancreatic Cancer Stem Cells Under in vitro and in vivo Conditions
DC Field | Value | Language |
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dc.contributor.author | 금웅섭 | - |
dc.date.accessioned | 2021-12-28T17:24:52Z | - |
dc.date.available | 2021-12-28T17:24:52Z | - |
dc.date.issued | 2021-09 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/187087 | - |
dc.description.abstract | Purpose: The study investigated the molecular mechanisms that killed pancreatic cancer cells, including cancer stem cells (CSCs), by carbon ion beam irradiation alone or in combination with miRNA-200c under in vitro and in vivo conditions. Methods: Human pancreatic cancer (PC) cells, PANC1 and PK45, were treated with carbon-ion beam irradiation alone or in combination with microRNA-200c (miR-200c) mimic. Cell viability assay, colony and spheroid formation assay, quantitative real-time PCR analysis of apoptosis-, autophagy-, and angiogenesis-related gene expression, xenograft tumor control and histopathological analyses were performed. Results: The cell viability assay showed that transfection of the miRNA-200c (10 nM) mimic into pancreatic CSC (CD44+/ESA+) and non-CSC (CD44-/ESA-) significantly suppressed proliferation of both types of cell populations described above. Combining carbon-ion beam irradiation with the miRNA-200c mimic significantly reduced the colony as well as spheroid formation abilities compared to that observed with the treatment of carbon-ion beam alone or X-ray irradiation combined with the miRNA-200c mimic. Moreover, the combination of carbon ion beam irradiation and miRNA-200c mimic increased the expression of apoptosis-related gene BAX, autophagy-related genes Beclin-1 and p62, addition of gemcitabine (GEM) further enhanced the expression of these genes. In vivo data showed that carbon-ion beam irradiation in combination with the miRNA-200c mimic effectively suppressed xenograft tumor growth and significantly induced tumor necrosis and cavitation. Conclusion: The combination of miRNA-200c mimic and carbon ion beam irradiation may be powerful radiotherapy that significantly kills pancreatic cancer cells containing CSCs and enhances the effect of carbon-ion beam irradiation compared to carbon-ion beam irradiation alone. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Dove Medical Press | - |
dc.relation.isPartOf | ONCOTARGETS AND THERAPY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Carbon-Ion Beam Irradiation and the miR-200c Mimic Effectively Eradicate Pancreatic Cancer Stem Cells Under in vitro and in vivo Conditions | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiation Oncology (방사선종양학교실) | - |
dc.contributor.googleauthor | Sei Sai | - |
dc.contributor.googleauthor | Eun Ho Kim | - |
dc.contributor.googleauthor | Woong Sub Koom | - |
dc.contributor.googleauthor | Guillaume Vares | - |
dc.contributor.googleauthor | Masao Suzuki | - |
dc.contributor.googleauthor | Shigeru Yamada | - |
dc.contributor.googleauthor | Mitsuhiro Hayashi | - |
dc.identifier.doi | 10.2147/OTT.S311567 | - |
dc.contributor.localId | A00273 | - |
dc.relation.journalcode | J02422 | - |
dc.identifier.eissn | 1178-6930 | - |
dc.identifier.pmid | 34556996 | - |
dc.subject.keyword | carbon-ion beam | - |
dc.subject.keyword | miR-200c | - |
dc.subject.keyword | pancreatic cancer stem cell | - |
dc.contributor.alternativeName | Koom, Woong Sub | - |
dc.contributor.affiliatedAuthor | 금웅섭 | - |
dc.citation.volume | 14 | - |
dc.citation.startPage | 4749 | - |
dc.citation.endPage | 4760 | - |
dc.identifier.bibliographicCitation | ONCOTARGETS AND THERAPY, Vol.14 : 4749-4760, 2021-09 | - |
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