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Rapid and accurate clinical testing for COVID-19 by nicking and extension chain reaction system-based amplification (NESBA)
DC Field | Value | Language |
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dc.contributor.author | 용동은 | - |
dc.contributor.author | 이혁민 | - |
dc.contributor.author | 홍기호 | - |
dc.date.accessioned | 2021-12-28T16:59:26Z | - |
dc.date.available | 2021-12-28T16:59:26Z | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/186882 | - |
dc.description.abstract | We herein describe rapid and accurate clinical testing for COVID-19 by nicking and extension chain reaction system-based amplification (NESBA), an ultrasensitive version of NASBA. The primers to identify SARS-CoV-2 viral RNA were designed to additionally contain the nicking recognition sequence at the 5'-end of conventional NASBA primers, which would enable nicking enzyme-aided exponential amplification of T7 RNA promoter-containing double-stranded DNA (T7DNA). As a consequence of this substantially enhanced amplification power, the NESBA technique was able to ultrasensitively detect SARS-CoV-2 genomic RNA (gRNA) down to 0.5 copies/μL (= 10 copies/reaction) for both envelope (E) and nucleocapsid (N) genes within 30 min under isothermal temperature (41 °C). When the NESBA was applied to test a large cohort of clinical samples (n = 98), the results fully agreed with those from qRT-PCR and showed the excellent accuracy by yielding 100% clinical sensitivity and specificity. By employing multiple molecular beacons with different fluorophore labels, the NESBA was further modulated to achieve multiplex molecular diagnostics, so that the E and N genes of SARS-CoV-2 gRNA were simultaneously assayed in one-pot. By offering the superior analytical performances over the current qRT-PCR, the isothermal NESBA technique could serve as very powerful platform technology to realize the point-of-care (POC) diagnosis for COVID-19. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Elsevier Advanced Technology | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Rapid and accurate clinical testing for COVID-19 by nicking and extension chain reaction system-based amplification (NESBA) | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Yong Ju | - |
dc.contributor.googleauthor | Jaemin Kim | - |
dc.contributor.googleauthor | Yeonkyung Park | - |
dc.contributor.googleauthor | Chang Yeol Lee | - |
dc.contributor.googleauthor | Kyungnam Kim | - |
dc.contributor.googleauthor | Ki Ho Hong | - |
dc.contributor.googleauthor | Hyukmin Lee | - |
dc.contributor.googleauthor | Dongeun Yong | - |
dc.contributor.googleauthor | Hyun Gyu Park | - |
dc.identifier.doi | 10.1016/j.bios.2021.113689 | - |
dc.contributor.localId | A02423 | - |
dc.contributor.localId | A03286 | - |
dc.relation.journalcode | J00330 | - |
dc.identifier.eissn | 1873-4235 | - |
dc.identifier.pmid | 34688112 | - |
dc.subject.keyword | COVID-19 | - |
dc.subject.keyword | Isothermal amplification | - |
dc.subject.keyword | NASBA | - |
dc.subject.keyword | NESBA | - |
dc.subject.keyword | SARS-CoV-2 | - |
dc.subject.keyword | qRT-PCR | - |
dc.contributor.alternativeName | Yong, Dong Eun | - |
dc.contributor.affiliatedAuthor | 용동은 | - |
dc.contributor.affiliatedAuthor | 이혁민 | - |
dc.citation.volume | 196 | - |
dc.citation.startPage | 113689 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, Vol.196 : 113689, 2022-01 | - |
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