타액반 피검물에서 개인식별을 위한 DNA의 유전자형 검사

Abstract

Cigarette butts from 5 smokers were gathered and then, placed in room temperature for 1,3, 5, 7,15 days. the possible use of the cigarette butts for individual indentification was evaluated in sex determination, amplification of D1S80 locus, polymorphisms of HLA-DQA1 goen from the extracted DNA. 1. DNA extraction was possible in cigarette butts left in room temperature for15 days, so it can be applicable to individual identification by polymerase chain reaction (PCR). 2. Amplification of X-Y homologous amelogenin gene by PCR made it possible to identify the sex in saliva stains(cigarette butts). 3. Amplification of D1S80 locus can be acquired from adding the bovine serum albimine and hot start PCR procedures from forensic samples such as saliva stains cigarette butts), so the AMP-FLPs examining is possible. 4. Genotype could be determined simply and rapidly using AmplitypeTMHLA-DQαorensic kit in examining the HLA-DQA1 gene. From the investigation, DNA extraction, sex determination, amplification of D1S80 ocus, polymorphisms of HLA DQA1 gene were successfully done even though the cigarette buts were left for 15 days at room temperature. Therefore cigarette butts rehighly reliable and applicable as molecular biological samples for individual dentification.