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Cytotoxicity of a cysteine proteinase of adult Clonorchis sinensis

Other Titles
 간흡충의 cysteine 단백분해효소의 세포독성 
Authors
 Hyun PARK  ;  Man Young KO  ;  Moon Kee PAIK  ;  Chin Thack SOH  ;  Jang Hoon SEO  ;  Kyung-il IM 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.33(3) : 211-218, 1995-09 
Journal Title
KOREAN JOURNAL OF PARASITOLOGY
ISSN
 0023-4001 
Issue Date
1995-09
MeSH
Animals ; CHO Cells / drug effects* ; Cells, Cultured ; Clonorchis sinensis / enzymology* ; Clonorchis sinensis / pathogenicity ; Cricetinae ; Cysteine Endopeptidases / isolation & purification ; Cysteine Endopeptidases / toxicity* ; Dose-Response Relationship, Drug ; Hydrogen-Ion Concentration ; Molecular Weight ; Rabbits
Abstract
To clarify the correlation of the proteinase activity with pathogenicity of Clonorchis sinensis, the proteinase activity either in excretory-secretory products (ESP) or in crude extracts of adult C. sinensis was examined. Substrate gel electrophoresis of the ESP and crude extracts revealed four distinct enzyme bands, which were differently inhibited by the specific proteinase inhibitors. The proteinase of the ESP with molecular mass of 24 kDa, was purified 23- fold with 14.5% yield by spectra gel ACA 44 gel filtration. It exhibited optimal pH at 7.5 in sodium phosphate (0.1 M). Its activity was inhibited specifically by N-ethylmaleimide (NEM) and antipain whereas potentiated 1.9 folds in the presence of 5 mM dithiothreitol (DTT). Cytotoxicity of the proteinase increased in a dose-dependent manner up to 120 µg/ml while reduced by NEM and antipain, indicating that cysteine proteinase was responsible for the cytotoxicity. This result shows that the 24 kDa cysteine proteinase is deeply correlated with the pathogenicity of C. sinensis infection.
Files in This Item:
T199501425.pdf Download
DOI
10.3347/kjp.1995.33.3.211
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Im, Kyung Il(임경일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/186384
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