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Axenization of Entamoeba histolytica, a Korean strain YS-27
DC Field | Value | Language |
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dc.contributor.author | 임경일 | - |
dc.date.accessioned | 2021-12-27T16:41:27Z | - |
dc.date.available | 2021-12-27T16:41:27Z | - |
dc.date.issued | 1995-12 | - |
dc.identifier.issn | 0023-4001 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/186062 | - |
dc.description.abstract | In the present communication, YS-27, a Korean strain of Entamoeba histolytica is described for the isolation and establishment of axenic cultivation. E. histolytica, designated as strain "YS-27", was isolated from the pus of a hepatic abscess obtained from a 72 year old inpatient of August 10, 1969. Specimens, were obtained by needle aspiration, inoculated immediately and weekly cultured in a modified diphasic medium at 37℃. Strain YS-27 had been maintained for more than 15 years by weekly subculture until February, 1985. These cultures were transferred to a monoxenic TTY-SB medium seeded with a trypanosomatid of the genus Crithidia. Penicillin G, 2 to 10 × 103 International units and Streptomycin, 2 to 10 mg per 100 ml, were added to the cultures to eliminate the bacteria. After more than one year later, these two organisms were well maintained by transfer every 3 or 4 days until January, 1986 at 37℃ in TTY-SB medium in the absence of other microorganisms. These monoxenic cultures were then transferred to TYI-S-33 medium. Strain YS-27 alone had not been growing at the time of transfer, but when overlaid with Crithidia at intervals of 3 to 4 days, strain YS-27 propagated well. The Crithidia died out several weeks later after several passages. Beginning in April, 1986, strain YS-27, was successfully established in axenic culture in TYI-S-33 medium and has been maintained in continuous culture and multiplied well to present. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English, Korean | - |
dc.publisher | Korean Society for Parasitology | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PARASITOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Culture Media | - |
dc.subject.MESH | Entamoeba histolytica / growth & development | - |
dc.subject.MESH | Entamoeba histolytica / isolation & purification* | - |
dc.subject.MESH | Humans | - |
dc.title | Axenization of Entamoeba histolytica, a Korean strain YS-27 | - |
dc.title.alternative | 이질아메바 한국분리주 YS-27의 무균 배양화 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Tropica Medicine (열대의학교실) | - |
dc.contributor.googleauthor | Jae-Kyung Chang | - |
dc.contributor.googleauthor | Kyung-il Im | - |
dc.contributor.googleauthor | Chin-Thack Soh | - |
dc.identifier.doi | 10.3347/kjp.1995.33.4.387 | - |
dc.contributor.localId | A03355 | - |
dc.relation.journalcode | J01957 | - |
dc.identifier.eissn | 1738-0006 | - |
dc.identifier.pmid | 8591018 | - |
dc.contributor.alternativeName | Im, Kyung Il | - |
dc.contributor.affiliatedAuthor | 임경일 | - |
dc.citation.volume | 33 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 387 | - |
dc.citation.endPage | 390 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PARASITOLOGY, Vol.33(4) : 387-390, 1995-12 | - |
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