Cited 78 times in
Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis
DC Field | Value | Language |
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dc.contributor.author | 최윤락 | - |
dc.date.accessioned | 2021-09-29T02:21:30Z | - |
dc.date.available | 2021-09-29T02:21:30Z | - |
dc.date.issued | 2021-01 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/184847 | - |
dc.description.abstract | The therapeutic application of human induced pluripotent stem cells (hiPSCs) for cartilage regeneration is largely hindered by the low yield of chondrocytes accompanied by unpredictable and heterogeneous off-target differentiation of cells during chondrogenesis. Here, we combine bulk RNA sequencing, single cell RNA sequencing, and bioinformatic analyses, including weighted gene co-expression analysis (WGCNA), to investigate the gene regulatory networks regulating hiPSC differentiation under chondrogenic conditions. We identify specific WNTs and MITF as hub genes governing the generation of off-target differentiation into neural cells and melanocytes during hiPSC chondrogenesis. With heterocellular signaling models, we further show that WNT signaling produced by off-target cells is responsible for inducing chondrocyte hypertrophy. By targeting WNTs and MITF, we eliminate these cell lineages, significantly enhancing the yield and homogeneity of hiPSC-derived chondrocytes. Collectively, our findings identify the trajectories and molecular mechanisms governing cell fate decision in hiPSC chondrogenesis, as well as dynamic transcriptome profiles orchestrating chondrocyte proliferation and differentiation. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Nature Pub. Group | - |
dc.relation.isPartOf | NATURE COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Benzeneacetamides / pharmacology | - |
dc.subject.MESH | Cell Differentiation / drug effects | - |
dc.subject.MESH | Cell Differentiation / genetics | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Chondrogenesis / drug effects | - |
dc.subject.MESH | Chondrogenesis / genetics* | - |
dc.subject.MESH | Computational Biology / methods | - |
dc.subject.MESH | Gene Expression Profiling / methods | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Induced Pluripotent Stem Cells / cytology | - |
dc.subject.MESH | Induced Pluripotent Stem Cells / metabolism | - |
dc.subject.MESH | Mice, Inbred NOD | - |
dc.subject.MESH | Mice, Knockout | - |
dc.subject.MESH | Mice, SCID | - |
dc.subject.MESH | Pluripotent Stem Cells / cytology | - |
dc.subject.MESH | Pluripotent Stem Cells / metabolism* | - |
dc.subject.MESH | Pyridines / pharmacology | - |
dc.subject.MESH | Single-Cell Analysis / methods* | - |
dc.subject.MESH | Transcriptome / drug effects | - |
dc.subject.MESH | Transcriptome / genetics* | - |
dc.title | Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학교실) | - |
dc.contributor.googleauthor | Chia-Lung Wu | - |
dc.contributor.googleauthor | Amanda Dicks | - |
dc.contributor.googleauthor | Nancy Steward | - |
dc.contributor.googleauthor | Ruhang Tang | - |
dc.contributor.googleauthor | Dakota B Katz | - |
dc.contributor.googleauthor | Yun-Rak Cho | - |
dc.contributor.googleauthor | Farshid Guilak | - |
dc.identifier.doi | 10.1038/s41467-020-20598-y | - |
dc.contributor.localId | A04136 | - |
dc.relation.journalcode | J02293 | - |
dc.identifier.eissn | 2041-1723 | - |
dc.identifier.pmid | 33441552 | - |
dc.contributor.alternativeName | Choi, Yun Rak | - |
dc.contributor.affiliatedAuthor | 최윤락 | - |
dc.citation.volume | 12 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 362 | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, Vol.12(1) : 362, 2021-01 | - |
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