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Exosome-based delivery of super-repressor IκBα ameliorates kidney ischemia-reperfusion injury

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dc.contributor.author강신욱-
dc.contributor.author김남희-
dc.contributor.author김현실-
dc.contributor.author박정탁-
dc.contributor.author유태현-
dc.contributor.author육종인-
dc.contributor.author한승혁-
dc.contributor.author김남희-
dc.contributor.author김현실-
dc.contributor.author박정탁-
dc.contributor.author유태현-
dc.contributor.author육종인-
dc.contributor.author한승혁-
dc.contributor.author김성훈-
dc.contributor.author남보영-
dc.date.accessioned2021-09-29T02:13:00Z-
dc.date.available2021-09-29T02:13:00Z-
dc.date.issued2021-09-
dc.identifier.issn0085-2538-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/184772-
dc.description.abstractIschemia-reperfusion injury is a major cause of acute kidney injury. Recent studies on the pathophysiology of ischemia-reperfusion-induced acute kidney injury showed that immunologic responses significantly affect kidney ischemia-reperfusion injury and repair. Nuclear factor (NF)-ĸB signaling, which controls cytokine production and cell survival, is significantly involved in ischemia-reperfusion-induced acute kidney injury, and its inhibition can ameliorate ischemic acute kidney injury. Using EXPLOR, a novel, optogenetically engineered exosome technology, we successfully delivered the exosomal super-repressor inhibitor of NF-ĸB (Exo-srIĸB) into B6 wild type mice before/after kidney ischemia-reperfusion surgery, and compared outcomes with those of a control exosome (Exo-Naïve)-injected group. Exo-srIĸB treatment resulted in lower levels of serum blood urea nitrogen, creatinine, and neutrophil gelatinase-associated lipocalin in post-ischemic mice than in the Exo-Naïve treatment group. Systemic delivery of Exo-srIĸB decreased NF-ĸB activity in post-ischemic kidneys and reduced apoptosis. Post-ischemic kidneys showed decreased gene expression of pro-inflammatory cytokines and adhesion molecules with Exo-srIĸB treatment as compared with the control. Intravital imaging confirmed the uptake of exosomes in neutrophils and macrophages. Exo-srIĸB treatment also significantly affected post-ischemic kidney immune cell populations, lowering neutrophil, monocyte/macrophage, and T cell frequencies than those in the control. Thus, modulation of NF-ĸB signaling through exosomal delivery can be used as a novel therapeutic method for ischemia-reperfusion-induced acute kidney injury.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfKIDNEY INTERNATIONAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAcute Kidney Injury* / etiology-
dc.subject.MESHAcute Kidney Injury* / prevention & control-
dc.subject.MESHAnimals-
dc.subject.MESHExosomes*-
dc.subject.MESHKidney-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHNF-KappaB Inhibitor alpha-
dc.subject.MESHReperfusion Injury* / prevention & control-
dc.titleExosome-based delivery of super-repressor IκBα ameliorates kidney ischemia-reperfusion injury-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.googleauthorSeonghun Kim-
dc.contributor.googleauthorSul A Lee-
dc.contributor.googleauthorHeakyung Yoon-
dc.contributor.googleauthorMyung Yoon Kim-
dc.contributor.googleauthorJae-Kwang Yoo-
dc.contributor.googleauthorSo-Hee Ahn-
dc.contributor.googleauthorCheol Hyoung Park-
dc.contributor.googleauthorJimin Park-
dc.contributor.googleauthorBo Young Nam-
dc.contributor.googleauthorJung Tak Park-
dc.contributor.googleauthorSeung Hyeok Han-
dc.contributor.googleauthorShin-Wook Kang-
dc.contributor.googleauthorNam Hee Kim-
dc.contributor.googleauthorHyun Sil Kim-
dc.contributor.googleauthorDawool Han-
dc.contributor.googleauthorJong In Yook-
dc.contributor.googleauthorChulhee Choi-
dc.contributor.googleauthorTae-Hyun Yoo-
dc.identifier.doi10.1016/j.kint.2021.04.039-
dc.contributor.localIdA00053-
dc.contributor.localIdA00360-
dc.contributor.localIdA01121-
dc.contributor.localIdA01654-
dc.contributor.localIdA02526-
dc.contributor.localIdA02536-
dc.contributor.localIdA04304-
dc.contributor.localIdA00360-
dc.contributor.localIdA01121-
dc.contributor.localIdA01654-
dc.contributor.localIdA02526-
dc.contributor.localIdA02536-
dc.contributor.localIdA04304-
dc.relation.journalcodeJ01941-
dc.identifier.eissn1523-1755-
dc.identifier.pmid34051264-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0085253821005081-
dc.subject.keywordNF-ĸB signaling-
dc.subject.keywordacute kidney injury-
dc.subject.keywordapoptosis-
dc.subject.keywordexosome-
dc.subject.keywordinflammation-
dc.subject.keywordischemia-reperfusion injury-
dc.contributor.alternativeNameKang, Shin Wook-
dc.contributor.affiliatedAuthor강신욱-
dc.contributor.affiliatedAuthor김남희-
dc.contributor.affiliatedAuthor김현실-
dc.contributor.affiliatedAuthor박정탁-
dc.contributor.affiliatedAuthor유태현-
dc.contributor.affiliatedAuthor육종인-
dc.contributor.affiliatedAuthor한승혁-
dc.contributor.affiliatedAuthor김남희-
dc.contributor.affiliatedAuthor김현실-
dc.contributor.affiliatedAuthor박정탁-
dc.contributor.affiliatedAuthor유태현-
dc.contributor.affiliatedAuthor육종인-
dc.contributor.affiliatedAuthor한승혁-
dc.citation.volume100-
dc.citation.number3-
dc.citation.startPage570-
dc.citation.endPage584-
dc.identifier.bibliographicCitationKIDNEY INTERNATIONAL, Vol.100(3) : 570-584, 2021-09-
Appears in Collections:
2. College of Dentistry (치과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers

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