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Measuring the absolute abundance of the microbiome by adding yeast containing 16S rRNA gene from a hyperthermophile

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dc.contributor.author김주영-
dc.contributor.author용동은-
dc.contributor.author용태순-
dc.contributor.author이명희-
dc.date.accessioned2021-09-29T02:08:46Z-
dc.date.available2021-09-29T02:08:46Z-
dc.date.issued2021-08-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/184735-
dc.description.abstractHigh-throughput sequencing (HTS) of 16S rRNA gene amplicons provides compo sitional information regarding the microbial community, but not the absolute abun dance of the bacteria. We aimed to develop a standardized method for quantifying the absolute abundance of bacteria in microbiome studies. To demonstrate the utility of our approach, we quantified the number of bacteria from the compositional data of the fecal and cecal microbiomes. The 16S rRNA gene of a hyperthermophile, Thermus aquaticus, was cloned into Pichia pastoris (yeast) genome, and an equivalent amount of the yeast was added to the stool and cecal samples of mice before DNA extraction. 16S rRNA gene library construction and HTS were performed after DNA extraction. The absolute abundances of bacteria were calculated using T. aquaticus reads. The average relative abundances of T. aquaticus in the five stool and five cecal samples were 0.95% and 0.33%, respectively, indicating that the number of bacteria in a cecum sample is 2.9 times higher than that in a stool sample. The method proposed for quan tifying the absolute abundance of the bacterial population in this study is expected to overcome the limitation of showing only compositional data in most microbiome studies.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherWiley-Blackwell-
dc.relation.isPartOfMICROBIOLOGYOPEN-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleMeasuring the absolute abundance of the microbiome by adding yeast containing 16S rRNA gene from a hyperthermophile-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Tropica Medicine (열대의학교실)-
dc.contributor.googleauthorJu Yeong Kim-
dc.contributor.googleauthorMyung-hee Yi-
dc.contributor.googleauthorMyungjun Kim-
dc.contributor.googleauthorSeogwon Lee-
dc.contributor.googleauthorHye Su Moon-
dc.contributor.googleauthorDongeun Yong-
dc.contributor.googleauthorTai-Soon Yong-
dc.identifier.doi10.1002/mbo3.1220-
dc.contributor.localIdA00937-
dc.contributor.localIdA02423-
dc.contributor.localIdA02424-
dc.contributor.localIdA02761-
dc.relation.journalcodeJ03569-
dc.identifier.eissn2045-8827-
dc.subject.keyword16S rRNA gene-
dc.subject.keywordabsolute abundance-
dc.subject.keywordhyperthermophile-
dc.subject.keywordmicrobiome-
dc.subject.keywordThermus aquaticus-
dc.contributor.alternativeNameKim, Ju Young-
dc.contributor.affiliatedAuthor김주영-
dc.contributor.affiliatedAuthor용동은-
dc.contributor.affiliatedAuthor용태순-
dc.contributor.affiliatedAuthor이명희-
dc.citation.volume10-
dc.citation.number4-
dc.citation.startPagee1220-
dc.identifier.bibliographicCitationMICROBIOLOGYOPEN, Vol.10(4) : e1220, 2021-08-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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