137 343

Cited 0 times in

Non-thermal atmospheric pressure plasma activates Wnt/β-catenin signaling in dermal papilla cells

DC Field Value Language
dc.contributor.author김도영-
dc.date.accessioned2021-09-29T02:07:22Z-
dc.date.available2021-09-29T02:07:22Z-
dc.date.issued2021-08-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/184723-
dc.description.abstractThere is an unmet need for novel, non-pharmacological therapeutics to treat alopecia. Recent studies have shown the potential biological benefits of non-thermal atmospheric pressure plasma (NTAPP), including wound healing, angiogenesis, and the proliferation of stem cells. We hypothesized that NTAPP might have a stimulatory effect on hair growth or regeneration. We designed an NTAPP-generating apparatus which is applicable to in vitro and in vivo experiments. The human dermal papilla (DP) cells, isolated fresh hair follicles, and mouse back skin were exposed with the NTAPP. Biological outcomes were measured using RNA-sequencing, RT-PCR, Western blots, and immunostaining. The NTAPP treatment increased the expression levels of Wnt/β-catenin pathway-related genes (AMER3, CCND1, LEF1, and LRG1) and proteins (β-catenin, p-GSK3β, and cyclin D1) in human DP cells. In contrast, inhibitors of Wnt/β-catenin signaling, endo-IWR1 and IWP2, attenuated the levels of cyclin D1, p-GSK3β, and β-catenin proteins induced by NTAPP. Furthermore, we observed that NTAPP induced the activation of β-catenin in DP cells of hair follicles and the mRNA levels of target genes of the β-catenin signaling pathway (CCND1, LEF1, and TCF4). NTAPP-treated mice exhibited markedly increased anagen induction, hair growth, and the protein levels of β-catenin, p-GSK3β, p-AKT, and cyclin D1. NTAPP stimulates hair growth via activation of the Wnt/β-catenin signaling pathway in DP cells. These findings collectively suggest that NTAPP may be a potentially safe and non-pharmacological therapeutic intervention for alopecia.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherNature Publishing Group-
dc.relation.isPartOfSCIENTIFIC REPORTS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleNon-thermal atmospheric pressure plasma activates Wnt/β-catenin signaling in dermal papilla cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Dermatology (피부과학교실)-
dc.contributor.googleauthorJi-Hye Hwang-
dc.contributor.googleauthorHyun-Young Lee-
dc.contributor.googleauthorKyung Bae Chung-
dc.contributor.googleauthorHae June Lee-
dc.contributor.googleauthorJino Kim-
dc.contributor.googleauthorKiwon Song-
dc.contributor.googleauthorDo-Young Kim-
dc.identifier.doi10.1038/s41598-021-95650-y-
dc.contributor.localIdA00384-
dc.relation.journalcodeJ02646-
dc.identifier.eissn2045-2322-
dc.identifier.pmid34373562-
dc.contributor.alternativeNameKim, Do Young-
dc.contributor.affiliatedAuthor김도영-
dc.citation.volume11-
dc.citation.number1-
dc.citation.startPage16125-
dc.identifier.bibliographicCitationSCIENTIFIC REPORTS, Vol.11(1) : 16125, 2021-08-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.