0 463

Cited 2 times in

Comparison of antinuclear antibody profiles obtained using line immunoassay and fluorescence enzyme immunoassay

Authors
 Jaehyeok Jang  ;  Sinyoung Kim  ;  Hyon-Suk Kim  ;  Kyung-A Lee  ;  Jungyong Park  ;  Younhee Park 
Citation
 JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, Vol.49(6) : 1-11, 2021-06 
Journal Title
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
ISSN
 0300-0605 
Issue Date
2021-06
MeSH
Antibodies, Antinuclear* ; Antigens, Nuclear* ; Fluorescent Antibody Technique, Indirect ; Humans ; Immunoassay ; Immunoenzyme Techniques ; Sensitivity and Specificity
Keywords
EliA ENA ; LIA-ANA-Profile-17S ; Sjögren’s syndrome ; antinuclear antibody ; diagnostic ; line immunoassay ; systemic lupus erythematosus
Abstract
Objective: LIA-ANA-Profile-17S is a multiplex line immunoassay that simultaneously detects 17 antinuclear antibodies (ANAs) against extractable nuclear antigens (ENAs). We evaluated the utility of LIA-ANA-Profile-17S as a supplement to ANA indirect immunofluorescence (IIF) and EliA ENA (a fluorescence enzyme immunoassay) for diagnosis of ANA-associated rheumatic diseases.

Methods: Sera were collected from 245 patients referred for an ANA IIF test. LIA-ANA-Profile-17S results were compared with those of EliA ENA. The kappa coefficients, agreement rates, and diagnostic performance of these tests were assessed for systemic lupus erythematosus (SLE) and Sjögren's syndrome (SjS).

Results: We observed almost perfect interassay agreement for antibodies against Ro52/Ro60, CENP-B, and Scl-70 (kappa = 0.91, 0.97, and 1.00, respectively); strong agreement for anti-SS-B/La antibody (kappa = 0.81); and relatively low agreement for other antibodies, including those against dsDNA, Sm, RNP, and Jo-1. For SLE diagnosis, LIA-ANA-Profile-17S showed lower sensitivity and similar specificity compared with EliA ENA. The sensitivity and specificity of these two assays were similar for SjS diagnosis.

Conclusions: The specificity of LIA-ANA-Profile-17S was enhanced when combined with ANA IIF and was comparable with that of EliA ENA. LIA-ANA-Profile-17S showed relatively good agreement with EliA ENA. In combination with ANA IIF, these assays showed enhanced diagnostic performance.
Full Text
https://journals.sagepub.com/doi/10.1177/03000605211014390
DOI
10.1177/03000605211014390
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sin Young(김신영) ORCID logo https://orcid.org/0000-0002-2609-8945
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Park, Youn Hee(박윤희) ORCID logo https://orcid.org/0000-0001-8458-1495
Lee, Kyung A(이경아) ORCID logo https://orcid.org/0000-0001-5320-6705
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/184508
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links