Cited 17 times in
Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids
DC Field | Value | Language |
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dc.contributor.author | 김한상 | - |
dc.date.accessioned | 2021-09-29T01:37:40Z | - |
dc.date.available | 2021-09-29T01:37:40Z | - |
dc.date.issued | 2021-03 | - |
dc.identifier.issn | 2666-1667 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/184465 | - |
dc.description.abstract | We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as blood, lymphatic fluid, and bile. The diversity of these samples requires robust and highly reproducible protocols and refined isolation technology, such as asymmetric-flow field-flow fractionation (AF4). Our isolation protocol allows for preparation of EVPs for various downstream applications, including proteomic profiling. For complete details on the use and execution of this protocol, please refer to Hoshino et al. (2020). | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Cell Press | - |
dc.relation.isPartOf | STAR Protocols | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Linda Bojmar | - |
dc.contributor.googleauthor | Han Sang Kim | - |
dc.contributor.googleauthor | Gabriel C Tobias | - |
dc.contributor.googleauthor | Fanny A Pelissier Vatter | - |
dc.contributor.googleauthor | Serena Lucotti | - |
dc.contributor.googleauthor | Kofi Ennu Gyan | - |
dc.contributor.googleauthor | Candia M Kenific | - |
dc.contributor.googleauthor | Zurong Wan | - |
dc.contributor.googleauthor | Kyung-A Kim | - |
dc.contributor.googleauthor | DooA Kim | - |
dc.contributor.googleauthor | Jonathan Hernandez | - |
dc.contributor.googleauthor | Virginia Pascual | - |
dc.contributor.googleauthor | Todd E Heaton | - |
dc.contributor.googleauthor | Michael P La Quaglia | - |
dc.contributor.googleauthor | David Kelsen | - |
dc.contributor.googleauthor | Tanya M Trippett | - |
dc.contributor.googleauthor | David R Jones | - |
dc.contributor.googleauthor | William R Jarnagin | - |
dc.contributor.googleauthor | Irina R Matei | - |
dc.contributor.googleauthor | Haiying Zhang | - |
dc.contributor.googleauthor | Ayuko Hoshino | - |
dc.contributor.googleauthor | David Lyden | - |
dc.identifier.doi | 10.1016/j.xpro.2020.100225 | - |
dc.contributor.localId | A01098 | - |
dc.relation.journalcode | J04093 | - |
dc.identifier.pmid | 33786456 | - |
dc.subject.keyword | Cell Membrane | - |
dc.subject.keyword | Cell culture | - |
dc.subject.keyword | Cell isolation | - |
dc.subject.keyword | Exomeres | - |
dc.subject.keyword | Exosomes | - |
dc.subject.keyword | Extracellular vesicles and particles | - |
dc.subject.keyword | Isolation | - |
dc.contributor.alternativeName | Kim, Han Sang | - |
dc.contributor.affiliatedAuthor | 김한상 | - |
dc.citation.volume | 2 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 100225 | - |
dc.identifier.bibliographicCitation | STAR Protocols, Vol.2(1) : 100225, 2021-03 | - |
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