Cited 2 times in
Chimerism Assay Using Single Nucleotide Polymorphisms Adjacent and in Linkage-Disequilibrium Enables Sensitive Disease Relapse Monitoring after Hematopoietic Stem-Cell Transplantation
DC Field | Value | Language |
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dc.contributor.author | 신새암 | - |
dc.contributor.author | 이승태 | - |
dc.contributor.author | 최종락 | - |
dc.date.accessioned | 2021-09-29T01:18:45Z | - |
dc.date.available | 2021-09-29T01:18:45Z | - |
dc.date.issued | 2021-04 | - |
dc.identifier.issn | 0009-9147 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/184301 | - |
dc.description.abstract | Background: Short tandem repeat (STR)-based chimerism analysis has been widely used for chimerism monitoring after hematopoietic stem-cell transplantation (HSCT), but technical artifacts can be problematic. We designed a chimerism assay using single nucleotide polymorphisms (SNPs) adjacent and in linkage-disequilibrium (CASAL), which doubly checked for SNP pairs, and thus could reduce background errors and increase analytical sensitivity. Methods: CASAL targeted 84 SNP pairs within 10 bp distance and in perfect linkage-disequilibrium. Using undiluted and serially diluted samples, baseline error rates, and linearity was calculated. Clinical performance of CASAL was evaluated in comparison with a conventional STR assay, using 191 posttransplant samples from 42 patients with HSCT. Results: CASAL had ∼10 times lower baseline error rates compared to that of ordinary next-generation sequencing. Limit of detection and quantification of CASAL were estimated to be 0.09 and 0.39%, respectively, with a linear range of 0.1-100%. CASAL correlated well with STR assay (r2 = 0.99) and the higher sensitivity enabled detection of low-level recipient chimerism and earlier prediction of relapse. Conclusions: CASAL is a simple, analytically sensitive and accurate assay that can be used in clinical samples after HSCT with a higher performance compared to that of traditional assays. It should also be useful in other forensic and archeological testing. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | American Association For Clinical Chemistry | - |
dc.relation.isPartOf | CLINICAL CHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Chimerism Assay Using Single Nucleotide Polymorphisms Adjacent and in Linkage-Disequilibrium Enables Sensitive Disease Relapse Monitoring after Hematopoietic Stem-Cell Transplantation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | JinJu Kim | - |
dc.contributor.googleauthor | Woobin Yun | - |
dc.contributor.googleauthor | Yu Jin Park | - |
dc.contributor.googleauthor | Jieun Seo | - |
dc.contributor.googleauthor | Richard Dong Wook Lee | - |
dc.contributor.googleauthor | Saeam Shin | - |
dc.contributor.googleauthor | Hyun-Ji Lee | - |
dc.contributor.googleauthor | In Suk Kim | - |
dc.contributor.googleauthor | Jong Rak Choi | - |
dc.contributor.googleauthor | Seung-Tae Lee | - |
dc.identifier.doi | 10.1093/clinchem/hvab010 | - |
dc.contributor.localId | A02108 | - |
dc.contributor.localId | A04627 | - |
dc.contributor.localId | A04182 | - |
dc.relation.journalcode | J00566 | - |
dc.identifier.eissn | 1530-8561 | - |
dc.identifier.pmid | 33582770 | - |
dc.identifier.url | https://academic.oup.com/clinchem/article/67/5/781/6134872 | - |
dc.subject.keyword | SNP | - |
dc.subject.keyword | STR | - |
dc.subject.keyword | chimerism | - |
dc.subject.keyword | hematopoietic stem-cell transplantation | - |
dc.subject.keyword | next-generation sequencing | - |
dc.subject.keyword | transplantation monitoring | - |
dc.contributor.alternativeName | Shin, Saeam | - |
dc.contributor.affiliatedAuthor | 신새암 | - |
dc.contributor.affiliatedAuthor | 이승태 | - |
dc.contributor.affiliatedAuthor | 최종락 | - |
dc.citation.volume | 67 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 781 | - |
dc.citation.endPage | 787 | - |
dc.identifier.bibliographicCitation | CLINICAL CHEMISTRY, Vol.67(5) : 781-787, 2021-04 | - |
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