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False-positive reactivity of anti-human leukocyte antigen antibodies detected using the single-antigen bead assay

Authors
 Boyeon Kim  ;  Sinyoung Kim  ;  Younhee Park  ;  Hyon-Suk Kim 
Citation
 HUMAN IMMUNOLOGY, Vol.82(6) : 409-413, 2021-06 
Journal Title
HUMAN IMMUNOLOGY
ISSN
 0198-8859 
Issue Date
2021-06
Keywords
Allele frequency ; Antibodies ; Human leukocyte antigen ; Single-antigen bead assay ; Transplantation
Abstract
The single-antigen bead assay (SABA) demonstrates high sensitivity and specificity for detecting anti-human leukocyte antigen (HLA) antibodies. However, SABA may produce false-positive results for anti-HLA antibodies. Herein, we analyzed the data of patients with complement-dependent cytotoxic crossmatch-/flow cytometric crossmatch-/SABA+/- results to determine false-positive results for anti-HLA antibodies. We also determined the prevalence of false-positive results by comparing false-positive data from our laboratory and national allele frequency data obtained with high-resolution HLA typing. For HLA-A, -B, -C, and -DR, a ratio of positive frequency to allele frequency of ≥3 in our laboratory was considered a false-positive result. For HLA-DQA1/DQB1 and HLA-DPA1/DPB1, we considered the positive frequency of ≥3 as a false positive result due to lack of haplotype frequency data. SABA results from 284 patients (78.0%) demonstrated false reactivity. The antibody against HLA-C*17:01 displayed the highest frequency ratio (298.3). If false-positive reactivity is suspected, results should be confirmed using different methods. If confirmation tests are unfeasible, comparing the allele frequency with the positive rate of detected anti-HLA antibodies and using a ratio ≥3 may facilitate the interpretation of SABA results. The positive rate of anti-HLA antibodies can be validated using the HLA allele frequency of the population to determine false-positive results.
Full Text
https://www.sciencedirect.com/science/article/pii/S0198885921000653
DOI
10.1016/j.humimm.2021.03.002
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Boyeon(김보연)
Kim, Sin Young(김신영) ORCID logo https://orcid.org/0000-0002-2609-8945
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Park, Youn Hee(박윤희) ORCID logo https://orcid.org/0000-0001-8458-1495
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/184287
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