0 51

Cited 0 times in

Plasmon-enhanced fluorescence correlation spectroscopy for super-localized detection of nanoscale subcellular dynamics

Authors
 Hongki Lee  ;  Woo Joong Rhee  ;  Gwiyeong Moon  ;  Seongmin Im  ;  Taehwang Son  ;  Jeon-Soo Shin  ;  Donghyun Kim 
Citation
 BIOSENSORS & BIOELECTRONICS, Vol.184 : 113219, 2021-07 
Journal Title
BIOSENSORS & BIOELECTRONICS
ISSN
 0956-5663 
Issue Date
2021-07
MeSH
Biosensing Techniques* ; Diffusion ; HEK293 Cells ; Humans ; Microscopy, Fluorescence ; Spectrometry, Fluorescence
Keywords
Fluorescence correlation spectroscopy ; Lysosome ; Nanogap ; Plasmonic nanodimer arrays ; Plasmonics ; Super-localization
Abstract
In this report, we investigate plasmon-enhanced imaging fluorescence correlation spectroscopy (p-FCS). p-FCS takes advantage of extreme light confinement by localization at nanogap-based plasmonic nanodimer arrays (PNAs) for enhanced signal-to-noise ratio (SNR) and improved precision by registration with surface plasmon microscopy images. Theoretical results corroborate the enhancement by PNAs in the far-field. Near-field scanning optical microscopy was used to confirm near-field localization experimentally. Experimental confirmation was also conducted with fluorescent nanobeads. The concept was further applied to studying the diffusion dynamics of lysosomes in HEK293T cells stimulated by phorbol 12-myristate 13-acetate treatment. It was found that lysosomes demonstrate stronger super-diffusive behavior with relatively weaker sub-diffusion after stimulation. SNR measured of p-FCS was improved by 9.77 times over conventional FCS. This report is expected to serve as the foundation for an enhanced analytical tool to explore subcellular dynamics.
Full Text
https://www.sciencedirect.com/science/article/pii/S0956566321002566
DOI
10.1016/j.bios.2021.113219
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Shin, Jeon Soo(신전수) ORCID logo https://orcid.org/0000-0002-8294-3234
Rhee, Woo Joong(이우중)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/184178
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links