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Fast and sensitive delineation of brain tumor with clinically compatible moxifloxacin labeling and confocal microscopy
DC Field | Value | Language |
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dc.contributor.author | 김의현 | - |
dc.date.accessioned | 2021-09-29T00:28:10Z | - |
dc.date.available | 2021-09-29T00:28:10Z | - |
dc.date.issued | 2020-01 | - |
dc.identifier.issn | 1864-063X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/183848 | - |
dc.description.abstract | Delineation of brain tumor margins during surgery is critical to maximize tumor removal while preserving normal brain tissue to obtain optimal clinical outcomes. Although various imaging methods have been developed, they have limitations to be used in clinical practice. We developed a high-speed cellular imaging method by using clinically compatible moxifloxacin and confocal microscopy for sensitive brain tumor detection and delineation. Moxifloxacin is a Food and Drug Administration (FDA) approved antibiotic and was used as a cell labeling agent through topical administration. Its strong fluorescence at short visible excitation wavelengths allowed video-rate cellular imaging. Moxifloxacin-based confocal microscopy (MBCM) was characterized in normal mouse brain specimens and visualized their cytoarchitecture clearly. Then, MBCM was applied to both brain tumor murine models and two malignant human brain tumors of glioblastoma and metastatic cancer. MBCM detected tumors in all the specimens by visualizing dense and irregular cell distributions, and tumor margins were easily delineated based on the cytoarchitecture. An image analysis method was developed for automated detection and delineation. MBCM demonstrated sensitive delineation of brain tumors through cytoarchitecture visualization and would have potentials for human applications, such as a surgery-guiding method for tumor removal. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Wiley-VCH | - |
dc.relation.isPartOf | JOURNAL OF BIOPHOTONICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Brain Neoplasms* / diagnostic imaging | - |
dc.subject.MESH | Glioblastoma* / diagnostic imaging | - |
dc.subject.MESH | Image Processing, Computer-Assisted | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Microscopy, Confocal | - |
dc.subject.MESH | Moxifloxacin | - |
dc.title | Fast and sensitive delineation of brain tumor with clinically compatible moxifloxacin labeling and confocal microscopy | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurosurgery (신경외과학교실) | - |
dc.contributor.googleauthor | Seunghun Lee | - |
dc.contributor.googleauthor | Won Yeong Park | - |
dc.contributor.googleauthor | Hoonchul Chang | - |
dc.contributor.googleauthor | Bumju Kim | - |
dc.contributor.googleauthor | Won Hyuk Jang | - |
dc.contributor.googleauthor | Seonghan Kim | - |
dc.contributor.googleauthor | Younghoon Shin | - |
dc.contributor.googleauthor | Myoung Joon Kim | - |
dc.contributor.googleauthor | Kyung Hwa Lee | - |
dc.contributor.googleauthor | Eui Hyun Kim | - |
dc.contributor.googleauthor | Euiheon Chung | - |
dc.contributor.googleauthor | Ki Hean Kim | - |
dc.identifier.doi | 10.1002/jbio.201900197 | - |
dc.contributor.localId | A00837 | - |
dc.relation.journalcode | J01271 | - |
dc.identifier.eissn | 1864-0648 | - |
dc.identifier.pmid | 31368257 | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/jbio.201900197 | - |
dc.subject.keyword | anti-bacterial agents | - |
dc.subject.keyword | brain neoplasms | - |
dc.subject.keyword | confocal microscopy | - |
dc.subject.keyword | fluorescence | - |
dc.subject.keyword | topical administration | - |
dc.contributor.alternativeName | Kim, Eui Hyun | - |
dc.contributor.affiliatedAuthor | 김의현 | - |
dc.citation.volume | 13 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | e201900197 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOPHOTONICS, Vol.13(1) : e201900197, 2020-01 | - |
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