매독균이 HaCaT세포의 표면항원 발현에 미치는 영향

Abstract

Background: In primary syphilis, Treponema pallidum invades skin and mucosa through the epidermis, enters blood vessels and circulates in the blood. Afterwards, T. pallidum migrates out of the blood vessels to the pidermis in secondary syphilis to induce diverse skin findings. Therefore, the epidermis seems to be one of the main targets in syphilis. Most of the epidermal cells are keratinocytes and it ca i be presumed that, T. pallidum infection elicit,s defenr.e mechanisms at kerat.inocyte level resulting in changes of the keratinocyt,e surface molecule expression. Objective .' We have cxamined by ELISA whether virulent T. pallidum and its 47-kDa antigen could change the expre.;sions of E cadherin, MHC I, and ICAM 1 on the surface of HaCaT, a transformed human kerai.inocyte cell line. Methods . The 96 well plates of 4 x 10' HaCaT cells/well were cultured for 1 2 days at 37g, and cultured for 16 hoi!rs at 37g including 3x 10'" T. pallidum per well or its 47-kDa antigen. Then the changes of E-cadherin, MHC I and, ICAM-I were observed by ELISA. Media alone was used for negative cont,ro: and TNF a of 10U/well was used for positive control. Results : On culturing with T. pallidum, the expression of E-cadherin and MHC I increased sig- nificantly compared with the negaiive control. That of ICAM 1 showed increased findings com- pared t,o the negative control, but, it was not, significant. On culturing with its 47 kDa antigen, the expression of E-cadioerin and MHC-I increased significantly compared with negative control. ICAM-1 expression was increased compared to the negative cont,rol, but it. was not significant,. Conclusion . The increase in E-cadherin and MHC I expressions in HaCaT cells cultured with T. pallidum may be related to the defence mechanisms of the host cells.