부분손상 백서 파킨슨씨병 모형의 Apomorphine 유발 회전운동과 tyrosine hydroxylase 면역조직화학적 염색 소견의 의의

Abstract

An apomorphine-induced rotational test has been used in the evaluation of rat parkinsonian models lesioned with neurotoxin 6-hydroxydopamine (6-OHDA). Previous parkinsonian rat models have generally been characterized by unilateral destruction of both nigrostriatal pathway and mesolimbic pathway using 6-OHDA. The authors created partial lesioned rat parkinsonian models using 6-OHDA in which there is destruction of the dopaminergic nigrostriatal pathway and sparing of the mesolimbic pathway. Rats with unilateral lesions of the substantia nigra pars compacta (SNpc) were tested for rotational asymmetry using a cylindrical rotameter device with flat bottom (diameter. 30.5cm) after administration of apomorphine. After completion of the rotation test. the animals were sacrificed and their brains were immunolabeled for tyrosine hydroxylase (TH). Analysis of anatomical and behavioral data suggests that the pattern of immunolabeled for tyrosine hydroxylase (TH). Analysis of anatomical and behavioral data suggests that the pattern of rotation (pivotal rotation) is a more reliable index for loss of TH-immunoreactive neurons in lesioned SNpc than the total number of rotational responses to apomorphine. The exact cause of the abnormal ipsiversive rotation which some rats showed is unclear. Further research should be pursued to explain this finding.