인체 대장상피세포에서의 Cytokine 유전자 발현 유도에 있어서 대장균과 이질 아메바 사이의 상호작용에 관한 연구

김정목; 정현채; 임경일; 조양자; 송인성; 김정룡

doi:Objective: Entamoeba histolytica induces secretion of interleukin-8 by cultured colon epithelial cells. However, expression of cytokine genes in colon epithelial cells exposed to E. histolytica in the presence of enteric bacteria are not reported at all. We assessed the array of cytokine genes expressed by colon epithelial cells in response to co-culture with E. histolytica trophozoites and tested the hypothesis that enteric bacteria may alter E. histolytica induced expression of cytokine genes. 
Methods: After HT-29 human colon carcinoma epithelial cells were co-cultured with E. histolytica trophozoites in the presence or absence of E. coli, RNA was extracted from the epithelial cells by a guanidinium thiocyanate-phenol-chloroform method. Cytokine gene expression was assessed by quantitative RT-PCR. 
Results: IL-8 mRNA was upregulated following exposure to E. histolytica and this was paralleled by increased IL-8 protein secretion. GM-CSF and IL-1a/βmRNAs were also upregulated in these cells. When HT-29 cells were co-cultured with E. coli DH5a and E. histolytica, there was a synergistic increase in IL-8 gene as well as IL-1a and GM-CSF expression. 
Conclusion: These results suggest that enteric bacteria significantly affect the early proinflammatory signals produced in host tissues in response to E. histolytica infection.

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인체 대장상피세포에서의 Cytokine 유전자 발현 유도에 있어서 대장균과 이질 아메바 사이의 상호작용에 관한 연구

DC Field	Value	Language
dc.contributor.author	임경일	-
dc.date.accessioned	2021-09-28T08:05:31Z	-
dc.date.available	2021-09-28T08:05:31Z	-
dc.date.issued	1996-06	-
dc.identifier.issn	0253-3162	-
dc.identifier.uri	https://ir.ymlib.yonsei.ac.kr/handle/22282913/183407	-
dc.description.statementOfResponsibility	restriction	-
dc.language	Korean	-
dc.publisher	Korean Society for Microbiology	-
dc.relation.isPartOf	Journal of the Korean Society for Microbiology(대한미생물학회지)	-
dc.rights	CC BY-NC-ND 2.0 KR	-
dc.title	인체 대장상피세포에서의 Cytokine 유전자 발현 유도에 있어서 대장균과 이질 아메바 사이의 상호작용에 관한 연구	-
dc.title.alternative	Study for Interaction between Escherichia coli and Entamoeba histolytica in Induction of Cytokine Gene Expression in Human Colon Epithelial Cells	-
dc.type	Article	-
dc.contributor.college	College of Medicine (의과대학)	-
dc.contributor.department	Dept. of Tropica Medicine (열대의학교실)	-
dc.contributor.googleauthor	김정목	-
dc.contributor.googleauthor	정현채	-
dc.contributor.googleauthor	임경일	-
dc.contributor.googleauthor	조양자	-
dc.contributor.googleauthor	송인성	-
dc.contributor.googleauthor	김정룡	-
dc.identifier.doi	Objective: Entamoeba histolytica induces secretion of interleukin-8 by cultured colon epithelial cells. However, expression of cytokine genes in colon epithelial cells exposed to E. histolytica in the presence of enteric bacteria are not reported at all. We assessed the array of cytokine genes expressed by colon epithelial cells in response to co-culture with E. histolytica trophozoites and tested the hypothesis that enteric bacteria may alter E. histolytica induced expression of cytokine genes. Methods: After HT-29 human colon carcinoma epithelial cells were co-cultured with E. histolytica trophozoites in the presence or absence of E. coli, RNA was extracted from the epithelial cells by a guanidinium thiocyanate-phenol-chloroform method. Cytokine gene expression was assessed by quantitative RT-PCR. Results: IL-8 mRNA was upregulated following exposure to E. histolytica and this was paralleled by increased IL-8 protein secretion. GM-CSF and IL-1a/βmRNAs were also upregulated in these cells. When HT-29 cells were co-cultured with E. coli DH5a and E. histolytica, there was a synergistic increase in IL-8 gene as well as IL-1a and GM-CSF expression. Conclusion: These results suggest that enteric bacteria significantly affect the early proinflammatory signals produced in host tissues in response to E. histolytica infection.	-
dc.contributor.localId	A03355	-
dc.relation.journalcode	J03751	-
dc.identifier.url	https://www.dbpia.co.kr/journal/articleDetail?nodeId=NODE01486747	-
dc.contributor.alternativeName	Im, Kyung Il	-
dc.contributor.affiliatedAuthor	임경일	-
dc.citation.volume	31	-
dc.citation.number	2	-
dc.citation.startPage	135	-
dc.citation.endPage	144	-
dc.identifier.bibliographicCitation	Journal of the Korean Society for Microbiology (대한미생물학회지), Vol.31(2) : 135-144, 1996-06	-

Appears in Collections:: 1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers

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