92 187

Cited 0 times in

Swelling of the vesicle is prerequisite for PTH secretion

 Sung Kil Lim  ;  Yi Hyun Kwon  ;  Young Duk Song  ;  Hyun Chul Lee  ;  Kyung Ja Ryu  ;  Kap Bum Huh  ;  Chun Sik Park 
 YONSEI MEDICAL JOURNAL, Vol.37(1) : 59-67, 1996-02 
Journal Title
Issue Date
Acetates / pharmacology ; Animals ; Body Fluids / metabolism* ; Cattle ; Cell Membrane Permeability ; Ionophores / pharmacology ; Mannitol / pharmacology ; Nigericin / pharmacology ; Osmosis ; Parathyroid Glands / drug effects ; Parathyroid Glands / metabolism* ; Parathyroid Hormone / metabolism*
Unlike most secretory cells, high extra cellular calcium inhibits rather than stimulates hormonal secretion in several cells such as parathyroid cells, Juxtaglomerular cells and osteoclast. To gain further insight into the common but unique stimulus-secretion coupling mechanism in these cells, bovine parathyroid slices were incubated in various conditions of Krebs-Ringer (KR) solution containing essential amino acids. Parathyroid cells showed the inverse dependency of secretion on extra cellular calcium concentration as we expected. Ammonium acetate overcame the inhibitory effect of 2.5 mM of calcium and the maximum effect was as much as the five times of the basal value, while there was a little additive effect under 0 mM CaCl2. PTH secretion was biphasic according to the change of extra cellular osmolarity and the lowest response was observed at 300 mOsm/l. In Na-rich KR solution, high concentration of nigericin (> 10-4M) completely overcame the inhibitory effect of 2.5 mM CaCl2 and the maximum stimulatory effect was 8 times greater whereas it was only 2 times greater without CaCl2. In K-rich KR solution that abolished the K-gradient between the extra cellular solution and the cytoplasm, the rate of PTH secretion increased, and furthermore the addition of nigericin increased the rate of secretion significantly. The results above suggested that the osmotic swelling of the secretory vesicle in parathyroid cells might promote exocytosis as in Juxtaglomerular cells. We propose that the swelling of the vesicle is also prerequisite for secretion in several cells inhibited paradoxically by Ca++, whatever the signal transduction pathway for swelling of the secretory granules induced by the lowering of Ca++ in cytoplasm are.
Files in This Item:
T199601083.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Lim, Sung Kil(임승길)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.