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A PCR-RFLP method for the detection of activated H-ras oncogene with a point mutation at codon 12 and 61
DC Field | Value | Language |
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dc.contributor.author | 정병하 | - |
dc.contributor.author | 홍성준 | - |
dc.date.accessioned | 2021-09-28T07:49:09Z | - |
dc.date.available | 2021-09-28T07:49:09Z | - |
dc.date.issued | 1996-12 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/183202 | - |
dc.description.abstract | To investigate the incidence of the H-ras gene activation in bladder tumor and the feasibility of using urinary washout samples for screening, a series of 33 human bladder tumors and their preoperatively collected urinary washout samples were screened using a mutant specific PCR-RFLP (polymerase chain-restriction fragment length polymorphism) to detect a point mutation of the H-ras gene. Five tumors were found to harbor H-ras mutations where two tumors had a glycine to valine (G-->T) change in codon 12 and three tumors had a glutamine to lysine (C-->A) change in codon 61, respectively. Moreover, we could also detect the same point mutations of the H-ras gene in corresponding urine washout samples. The incidence of H-ras mutation in Korean bladder cancer was estimated at approximately 15.2%. In conclusion, a mutant specific PCR-RFLP method for the detection of H-ras gene mutation is useful for screening or postoperative follow-up of bladder tumor due to its simplicity and high specificity even in urinary samples. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Carcinoma, Transitional Cell / genetics | - |
dc.subject.MESH | Codon | - |
dc.subject.MESH | Gene Expression Regulation* | - |
dc.subject.MESH | Genes, ras* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Point Mutation* | - |
dc.subject.MESH | Polymerase Chain Reaction* | - |
dc.subject.MESH | Polymorphism, Restriction Fragment Length* | - |
dc.subject.MESH | Transcriptional Activation | - |
dc.subject.MESH | Urinary Bladder Neoplasms / genetics | - |
dc.title | A PCR-RFLP method for the detection of activated H-ras oncogene with a point mutation at codon 12 and 61 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Urology (비뇨의학교실) | - |
dc.contributor.googleauthor | Sung Joon Hong | - |
dc.contributor.googleauthor | Tack Lee | - |
dc.contributor.googleauthor | Young Sug Park | - |
dc.contributor.googleauthor | Kyung Ok Lee | - |
dc.contributor.googleauthor | Byung Ha Chung | - |
dc.contributor.googleauthor | Soo Hyung Lee | - |
dc.identifier.doi | 10.3349/ymj.1996.37.6.371 | - |
dc.contributor.localId | A03607 | - |
dc.contributor.localId | A04402 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 9048488 | - |
dc.contributor.alternativeName | Chung, Byung Ha | - |
dc.contributor.affiliatedAuthor | 정병하 | - |
dc.contributor.affiliatedAuthor | 홍성준 | - |
dc.citation.volume | 37 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 371 | - |
dc.citation.endPage | 379 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.37(6) : 371-379, 1996-12 | - |
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