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흰쥐 호흡상피와 후각상피에서 미세혈관의 렉틴 결합반응

Other Titles
 Lectin-binding Patterns with Endothelial Cells of Murine Respiratory and Olfactory Mucosa 
Authors
 김경수  ;  이정권  ;  윤주헌  ;  박인용  ;  문형진 
Citation
 Journal of Rhinology (대한비과학회지), Vol.3 : 24-29, 1996-01 
Journal Title
Journal of Rhinology(대한비과학회지)
ISSN
 1225-6870 
Issue Date
1996-01
Keywords
Lectin ; Murine nasal mucosa ; Endothelium ; Marker ; Murine nasal mucosa Endothelium
Abstract
The objectives of this study were to observe the lectin-binding patterns of endothelial cells of murine respiratory and olfactory mucosa and to find the marker pectin differentiating fenestrated from continuous vessels on light microscopy. Twelve BALB/C mice were fixed with 10% buffered formalin and embedded in paraffin. Serial sections, 4 μm in thickness, were made and stained with sialic acid-specific pectins (WGA, MAA, SNA) and sialic acid-nonspecific pectins (RCA-I, DBA, GSI-B4, UEA-I, SBA, PNA, Con-A) using the avidin-biotin peroxidase method. Diamlnobenzidine was used as a chromogen. The slides were counterstained with the Mayer's hematoxylin. pectin-binding patterns were observed using Vanox-S light microscope. WGA and RCA-I were labelled on respiratory and olfactory mucosa irrespective of the type of blood vessel but the binding patterns with WGA were inhomogeneous. These results suggest that N-acetrlglucosamine, sialic acid, N-acetylgalactosamine, and galactose exist In endothelial surface of murine nasal mucosa. But we could not find the marker lectin which differentiates continuous from fenestrated vessels. Further study using the Lowicryl K4M-embedding method and immunoelectromicroscopy will be required.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Su(김경수) ORCID logo https://orcid.org/0000-0003-1460-0640
Yoon, Joo Heon(윤주헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/183191
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