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항고혈압 약제가 백서 사구체 메산지움 및 대동맥 평활근 배양세포의 DNA 합성 및 세포 증식에 미치는 영향
DC Field | Value | Language |
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dc.contributor.author | 최규헌 | - |
dc.date.accessioned | 2021-09-28T07:46:47Z | - |
dc.date.available | 2021-09-28T07:46:47Z | - |
dc.date.issued | 1996-01 | - |
dc.identifier.issn | 1225-8911 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/183174 | - |
dc.description.abstract | The aim of this study was to evaluate the effect of anti-hypertensive drugs, nifedipine, furosemide, and captopril, on DNA synthesis and proliferation of cultured rat glomerular mesangical and aortic smooth muscle cells induced by fetal calf serum(20% in mesangial cells and 10% in smooth muscle cells). Glomerular mesangial and aortic smooth muscle cells from Sprague-Dawley rats were cultured and seeded in multi-well plates. After confluency and serum restriction(48 hours in mesangial cells and 72 hours in smooth muscle cells), the cells were exposed to fetal calf serum with or without anti-hypertensive drugs at increasing concentration between 10^(-8) -10^(-4)M. DNA synthesis was assessed by ³H-thymidine uptake and proliferation by cell numbers using hemocytometer. The results were as follows: 1) Nifedipine at the concentration of 10^(-5)M and 5×10^(-4)M inhibited serum-induced DNA synthesis significantly by 40.9% and 78.9% in mesangial cells(p<0.05), and by 50.8% and 86.6% in smooth muscle cells(p><0.05), respectively. The results of cell numbers paralleled those of ³H-thymidine uptake. 2) Serum-induced DNA synthesis of mesangial and smooth muscle cells were reduced by 23.5% and 32.6%, respectively, at the highest dose of furosemide(10^(-4M) without statistial significance. 3) Neither hydrochlorothiazide nor captopril exhibited any anti-proliferative effect on mesangial and smooth muscle cells throughout the entire doses. In conclusion, among the various anti-hypertensive drugs, nifedipine appears to be beneficial in view of a direct inhibitory effect on DNA synthesis and proliferation of mesangial and smooth muscle cells besides of anti-hyertensive effect. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean | - |
dc.publisher | 대한고혈압학회 | - |
dc.relation.isPartOf | Journal of the Korean Society of Hypertension(대한고혈압학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | 항고혈압 약제가 백서 사구체 메산지움 및 대동맥 평활근 배양세포의 DNA 합성 및 세포 증식에 미치는 영향 | - |
dc.title.alternative | The Effect of Anti-Hypertensive Drugs on DNA Synthesis and Proliferation of Cultured Rat Glomerular Mesangial and Aoric Smooth Muscle Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | 강신욱 | - |
dc.contributor.googleauthor | 이인희 | - |
dc.contributor.googleauthor | 박종훈 | - |
dc.contributor.googleauthor | 최규헌 | - |
dc.contributor.googleauthor | 하성규 | - |
dc.contributor.googleauthor | 이호영 | - |
dc.contributor.localId | A04043 | - |
dc.relation.journalcode | J01874 | - |
dc.contributor.alternativeName | Choi, Kyu Hun | - |
dc.contributor.affiliatedAuthor | 최규헌 | - |
dc.citation.volume | 2 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 137 | - |
dc.citation.endPage | 148 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Society of Hypertension (대한고혈압학회지), Vol.2(2) : 137-148, 1996-01 | - |
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