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Three-Dimensional Regeneration of Patient-Derived Intestinal Organoid Epithelium in a Physiodynamic Mucosal Interface-on-a-Chip
DC Field | Value | Language |
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dc.contributor.author | 고홍 | - |
dc.date.accessioned | 2021-05-21T16:53:47Z | - |
dc.date.available | 2021-05-21T16:53:47Z | - |
dc.date.issued | 2020-07 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/182607 | - |
dc.description.abstract | The regeneration of the mucosal interface of the human intestine is critical in the host-gut microbiome crosstalk associated with gastrointestinal diseases. The biopsy-derived intestinal organoids provide genetic information of patients with physiological cytodifferentiation. However, the enclosed lumen and static culture condition substantially limit the utility of patient-derived organoids for microbiome-associated disease modeling. Here, we report a patient-specific three-dimensional (3D) physiodynamic mucosal interface-on-a-chip (PMI Chip) that provides a microphysiological intestinal milieu under defined biomechanics. The real-time imaging and computational simulation of the PMI Chip verified the recapitulation of non-linear luminal and microvascular flow that simulates the hydrodynamics in a living human gut. The multiaxial deformations in a convoluted microchannel not only induced dynamic cell strains but also enhanced particle mixing in the lumen microchannel. Under this physiodynamic condition, an organoid-derived epithelium obtained from the patients diagnosed with Crohn's disease, ulcerative colitis, or colorectal cancer independently formed 3D epithelial layers with disease-specific differentiations. Moreover, co-culture with the human fecal microbiome in an anoxic-oxic interface resulted in the formation of stochastic microcolonies without a loss of epithelial barrier function. We envision that the patient-specific PMI Chip that conveys genetic, epigenetic, and environmental factors of individual patients will potentially demonstrate the pathophysiological dynamics and complex host-microbiome crosstalk to target a patient-specific disease modeling. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Multidisciplinary Digital Publishing Institute | - |
dc.relation.isPartOf | MICROMACHINES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Three-Dimensional Regeneration of Patient-Derived Intestinal Organoid Epithelium in a Physiodynamic Mucosal Interface-on-a-Chip | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | ;Dept. of Pediatrics (소아과학교실) | - |
dc.contributor.googleauthor | Yong Cheol Shin | - |
dc.contributor.googleauthor | Woojung Shin | - |
dc.contributor.googleauthor | Domin Koh | - |
dc.contributor.googleauthor | Alexander Wu | - |
dc.contributor.googleauthor | Yoko M Ambrosini | - |
dc.contributor.googleauthor | Soyoun Min | - |
dc.contributor.googleauthor | S Gail Eckhardt | - |
dc.contributor.googleauthor | R Y Declan Fleming | - |
dc.contributor.googleauthor | Seung Kim | - |
dc.contributor.googleauthor | Sowon Park | - |
dc.contributor.googleauthor | Hong Koh | - |
dc.contributor.googleauthor | Tae Kyung Yoo | - |
dc.contributor.googleauthor | Hyun Jung Kim | - |
dc.identifier.doi | 10.3390/mi11070663 | - |
dc.contributor.localId | A00156 | - |
dc.relation.journalcode | J03026 | - |
dc.identifier.eissn | 2072-666X | - |
dc.identifier.pmid | 32645991 | - |
dc.subject.keyword | co-culture | - |
dc.subject.keyword | disease modeling | - |
dc.subject.keyword | gut-on-a-chip | - |
dc.subject.keyword | microbiome | - |
dc.subject.keyword | mucosal interface | - |
dc.subject.keyword | multiaxial deformation | - |
dc.subject.keyword | organoid | - |
dc.subject.keyword | physiodynamic | - |
dc.contributor.alternativeName | Koh, Hong | - |
dc.contributor.affiliatedAuthor | 고홍 | - |
dc.citation.volume | 11 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 663 | - |
dc.identifier.bibliographicCitation | MICROMACHINES, Vol.11(7) : 663, 2020-07 | - |
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