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High mobility group box-1 promotes inflammation in endometriotic stromal cells through Toll-like receptor 4/nuclear factor-kappa B
DC Field | Value | Language |
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dc.contributor.author | 김성훈 | - |
dc.contributor.author | 서석교 | - |
dc.contributor.author | 윤보현 | - |
dc.contributor.author | 이병석 | - |
dc.contributor.author | 조시현 | - |
dc.contributor.author | 최영식 | - |
dc.date.accessioned | 2021-04-29T17:39:44Z | - |
dc.date.available | 2021-04-29T17:39:44Z | - |
dc.date.issued | 2021-03 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/182457 | - |
dc.description.abstract | Objective: To investigate whether high-mobility group box-1 induces cell proliferation, invasion and mediates inflammation in ectopic human endometrial stromal cells through Toll-like receptor 4. Methods: Ectopic endometrial specimens were retrieved from patients with ovarian endometrioma having laparoscopy. Ectopic HESCs were treated with H2O2 and recombinant HMGB-1 to induce oxidative stress. The effect of oxidative stress on cell proliferation and invasion was demonstrated. Receptors for HMGB-1 in NF-κB pathway (TLR4, RAGE), angiogenic molecule (VEGF), adhesion molecules (ICAM-1, E-cadherin), and inflammatory cytokines were measured simultaneously to the oxidative stress. Results: Ectopic HESCs showed markedly decreased cell viability with the increased release of HMGB-1 following treatment with H2O2. When ectopic HESCs were stressed by rHMGB-1, cell proliferation and cell migration numbers increased significantly in a dose-dependent manner. Increased TLR4 and RAGE mRNA and protein expression levels were noted to rHMGB-1 treatment in a dose-dependent manner. VEGF synthesis was also increased by rHMGB-1 treatment. The gene expression of ICAM-1 was upregulated, whereas that of E-cadherin was downregulated with rHMGB-1 treatment. Interleukin-6, IL-1β, tumor necrosis factor-alpha, and IL-10 were increased significantly by rHMGB-1 treatment. Inversely, after transfection of small interfering RNA against TLR4, rHMGB treatment resulted in decreased cell proliferation and invasion. Conclusion: HMGB-1 activates the NF-κB pathway via TLR4 to increase cell proliferation, invasion, and the production of various inflammatory markers in HESCs. Thus, HMGB-1, TLR4, and NF-κB may represent potential therapeutic targets for the treatment of endometriosis. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | e-Century Pub. Corp | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | High mobility group box-1 promotes inflammation in endometriotic stromal cells through Toll-like receptor 4/nuclear factor-kappa B | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Obstetrics and Gynecology (산부인과학교실) | - |
dc.contributor.googleauthor | Bo Hyon Yun | - |
dc.contributor.googleauthor | Sunghoon Kim | - |
dc.contributor.googleauthor | Seung Joo Chon | - |
dc.contributor.googleauthor | Ga Hee Kim | - |
dc.contributor.googleauthor | Young Sik Choi | - |
dc.contributor.googleauthor | SiHyun Cho | - |
dc.contributor.googleauthor | Byung Seok Lee | - |
dc.contributor.googleauthor | Seok Kyo Seo | - |
dc.contributor.localId | A00595 | - |
dc.contributor.localId | A01888 | - |
dc.contributor.localId | A02555 | - |
dc.contributor.localId | A02795 | - |
dc.contributor.localId | A03846 | - |
dc.contributor.localId | A04114 | - |
dc.relation.journalcode | J02843 | - |
dc.identifier.eissn | 1943-8141 | - |
dc.identifier.pmid | 33841665 | - |
dc.subject.keyword | Endometriosis | - |
dc.subject.keyword | HMGB-1 | - |
dc.subject.keyword | TLR4 | - |
dc.subject.keyword | oxidative stress | - |
dc.contributor.alternativeName | Kim, Sung Hoon | - |
dc.contributor.affiliatedAuthor | 김성훈 | - |
dc.contributor.affiliatedAuthor | 서석교 | - |
dc.contributor.affiliatedAuthor | 윤보현 | - |
dc.contributor.affiliatedAuthor | 이병석 | - |
dc.contributor.affiliatedAuthor | 조시현 | - |
dc.contributor.affiliatedAuthor | 최영식 | - |
dc.citation.volume | 13 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1400 | - |
dc.citation.endPage | 1410 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH, Vol.13(3) : 1400-1410, 2021-03 | - |
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