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Massively parallel sequencing of 25 autosomal STRs including SE33 in four population groups for forensic applications
DC Field | Value | Language |
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dc.contributor.author | 신경진 | - |
dc.contributor.author | 이은영 | - |
dc.date.accessioned | 2021-04-29T17:20:47Z | - |
dc.date.available | 2021-04-29T17:20:47Z | - |
dc.date.issued | 2021-02 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/182296 | - |
dc.description.abstract | The introduction of massively parallel sequencing (MPS) in forensic investigation enables sequence-based large-scale multiplexing beyond size-based analysis using capillary electrophoresis (CE). For the practical application of MPS to forensic casework, many population studies have provided sequence data for autosomal short tandem repeats (STRs). However, SE33, a highly polymorphic STR marker, has little sequence-based data because of difficulties in analysis. In this study, 25 autosomal STRs were analyzed, including SE33, using an in-house MPS panel for 350 samples from four populations (African-American, Caucasian, Hispanic, and Korean). The barcoded MPS library was generated using a two-step PCR method and sequenced using a MiSeq System. As a result, 99.88% genotype concordance was obtained between length- and sequence-based analyses. In SE33, the most discordances (eight samples, 0.08%) were observed because of the 4 bp deletion between the CE and MPS primer binding sites. Compared with the length-based CE method, the number of alleles increased from 332 to 725 (2.18-fold) for 25 autosomal STRs in the sequence-based MPS method. Notably, additional 129 unique alleles, a 4.15-fold increase, were detected in SE33 by identifying sequence variations. This population data set provides sequence variations and sequence-based allele frequencies for 25 autosomal STRs. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Massively parallel sequencing of 25 autosomal STRs including SE33 in four population groups for forensic applications | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Forensic Medicine (법의학과) | - |
dc.contributor.googleauthor | Ye-Lim Kwon | - |
dc.contributor.googleauthor | Bo Min Kim | - |
dc.contributor.googleauthor | Eun Young Lee | - |
dc.contributor.googleauthor | Kyoung-Jin Shin | - |
dc.identifier.doi | 10.1038/s41598-021-82814-z | - |
dc.contributor.localId | A02085 | - |
dc.contributor.localId | A03041 | - |
dc.relation.journalcode | J02646 | - |
dc.identifier.eissn | 2045-2322 | - |
dc.identifier.pmid | 33633141 | - |
dc.contributor.alternativeName | Shin, Kyoung Jin | - |
dc.contributor.affiliatedAuthor | 신경진 | - |
dc.contributor.affiliatedAuthor | 이은영 | - |
dc.citation.volume | 11 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 4701 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, Vol.11(1) : 4701, 2021-02 | - |
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