0 19

Cited 0 times in

In vivo monitoring platform of transplanted human stem cells using magnetic resonance imaging

 Seungmin Han  ;  Byunghoon Kang  ;  Hye Young Son  ;  Yuna Choi  ;  Moo-Kwang Shin  ;  Jongjin Park  ;  Jeong-Ki Min  ;  Daewon Park  ;  Eun-Kyung Lim  ;  Yong-Min Huh  ;  Seungjoo Haam 
 BIOSENSORS & BIOELECTRONICS, Vol.178 : 113039, 2021-04 
Journal Title
Issue Date
Hollow MnO nanoparticle ; Human anti-integrin β1 antibody ; In vivo MR monitoring ; Stem cell monitoring ; Stem cell therapy
As stem cells show great promise in regenerative therapy, stem cell-mediated therapeutic efficacy must be demonstrated through the migration and transplantation of stem cells into target disease areas at the pre-clinical level. In this study, we developed manganese-based magnetic nanoparticles with hollow structures (MnOHo) and modified them with the anti-human integrin β1 antibody (MnOHo-Ab) to enable the minimal-invasive monitoring of transplanted human stem cells at the pre-clinical level. Compared to common magnetic resonance imaging (MRI)-based stem cell monitoring systems that use pre-labeled stem cells with magnetic particles before stem cell injection, the MnOHo-Ab is a new technology that does not require stem cell modification to monitor the therapeutic capability of stem cells. Additionally, MnOHo-Ab provides improved T1 MRI owing to the hollow structure of the MnOHo. Particularly, the anti-integrin β1 antibody (Ab) introduced in the MnOHo targets integrin β1 expressed in the entire stem cell lineage, enabling targeted monitoring regardless of the differentiation stage of the stem cells. Furthermore, we verified that intravenously injected MnOHo-Ab specifically targeted human induced pluripotent stem cells (hiPSCs) that were transferred to mice testes and differentiated into various lineages. The new stem cell monitoring method using MnOHo-Ab demonstrates whether the injected human stem cells have migrated and transplanted themselves in the target area during long-term stem cell regenerative therapy.
Full Text
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Son, Hye Yeong(손혜영) ORCID logo https://orcid.org/0000-0001-5977-6784
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.