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Diagnosis of severe sepsis using phospholipids enzymatic assay based on cyclic voltammetry

Authors
 Jun-Hee Park  ;  Jong-Min Park  ;  Ga-Yeon Lee  ;  Zhiquan Song  ;  Jeong Soo Sung  ;  Min-Jung Kang  ;  Nam Su Ku  ;  Eun Hye Lee  ;  Kwang Hwan Park  ;  Moo Suk Park  ;  Sang-Guk Lee  ;  Jae-Chul Pyun 
Citation
 ENZYME AND MICROBIAL TECHNOLOGY, Vol.144 : 109728, 2021-03 
Journal Title
ENZYME AND MICROBIAL TECHNOLOGY
ISSN
 0141-0229 
Issue Date
2021-03
Keywords
Cyclic voltammetry ; Diagnosis ; Enzymatic assay ; Graphite ; Phospholipid ; Sepsis
Abstract
In this work phospholipid quantification was carried out using an enzymatic assay based on cyclic voltammetry of the condensation product of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt (DAOS) and 4-aminoantipyrine (4-AP) with a graphite electrode. For the optimization of electrochemical measurement for the product, electrochemical properties such as the electrochemical window, double layer capacitance (Cdl) and electron transfer rate (kapp) were analyzed for a graphite-electrode and Au-electrode. The phospholipid enzymatic assay based the on electrochemical measurement using the graphite electrode was applied to the diagnosis of sepsis for sera from healthy volunteers (n = 16), patients with systemic inflammatory response syndrome (SIRS, n = 16) and severe sepsis patients (n = 24). Finally, the phospholipid quantification results from the electrochemical measurement were statistically compared with the conventional method based on optical density measurement.
Full Text
https://www.sciencedirect.com/science/article/pii/S0141022920302210
DOI
10.1016/j.enzmictec.2020.109728
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Ku, Nam Su(구남수) ORCID logo https://orcid.org/0000-0002-9717-4327
Park, Kwang Hwan(박광환) ORCID logo https://orcid.org/0000-0002-2110-0559
Park, Moo Suk(박무석) ORCID logo https://orcid.org/0000-0003-0820-7615
Lee, Sang-Guk(이상국) ORCID logo https://orcid.org/0000-0003-3862-3660
Lee, Eun Hye(이은혜) ORCID logo https://orcid.org/0000-0003-2570-3442
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/181973
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