Diagnosis of severe sepsis using phospholipids enzymatic assay based on cyclic voltammetry
Authors
Jun-Hee Park ; Jong-Min Park ; Ga-Yeon Lee ; Zhiquan Song ; Jeong Soo Sung ; Min-Jung Kang ; Nam Su Ku ; Eun Hye Lee ; Kwang Hwan Park ; Moo Suk Park ; Sang-Guk Lee ; Jae-Chul Pyun
Citation
ENZYME AND MICROBIAL TECHNOLOGY, Vol.144 : 109728, 2021-03
In this work phospholipid quantification was carried out using an enzymatic assay based on cyclic voltammetry of the condensation product of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt (DAOS) and 4-aminoantipyrine (4-AP) with a graphite electrode. For the optimization of electrochemical measurement for the product, electrochemical properties such as the electrochemical window, double layer capacitance (Cdl) and electron transfer rate (kapp) were analyzed for a graphite-electrode and Au-electrode. The phospholipid enzymatic assay based the on electrochemical measurement using the graphite electrode was applied to the diagnosis of sepsis for sera from healthy volunteers (n = 16), patients with systemic inflammatory response syndrome (SIRS, n = 16) and severe sepsis patients (n = 24). Finally, the phospholipid quantification results from the electrochemical measurement were statistically compared with the conventional method based on optical density measurement.