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Effects of Particulate Matter 10 Inhalation on Lung Tissue RNA expression in a Murine Model

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dc.contributor.authorHan, Heejae-
dc.contributor.authorOh, Eun-Yi-
dc.contributor.authorLee, Jae-Hyun-
dc.contributor.authorPark, Jung-Won-
dc.contributor.authorPark, Hye Jung-
dc.date.accessioned2021-03-31T02:10:43Z-
dc.date.available2021-03-31T02:10:43Z-
dc.date.created2021-02-22-
dc.date.issued2021-01-
dc.identifier.issn1738-3536-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/181871-
dc.description.abstractBackground: Particulate matter 10 (PM10; airborne particles <10 mu m) inhalation has been demonstrated to induce airway and lung diseases. In this study, we investigate the effects of PM10 inhalation on RNA expression in lung tissues using a murine model. Methods: Female BALB/c mice were affected with PM10, ovalbumin (OVA), or both OVA and PM10. PM10 was administered intranasally while OVA was both intraperitoneally injected and intranasally administered. Treatments occurred 4 times over a 2-week period. Two days after the final challenges, mice were sacrificed. Full RNA sequencing using lung homogenates was conducted. Results: While PM10 did not induce cell proliferation in bronchoalveolar fluid or lead to airway hyper-responsiveness, it did cause airway inflammation and lung fibrosis. Levels of interleukin 1 beta, tumor necrosis factor-alpha, and transforming growth factor-beta in lung homogenates were significantly elevated in the PM10-treated group, compared to the control group. The PM10 group also showed increased RNA expression of Rn45a, Snord22, Atp6v0c-ps2, Snora28, Snord15b, Snora70, and Mmp12. Generally, genes associated with RNA splicing, DNA repair, the inflammatory response, the immune response, cell death, and apoptotic processes were highly expressed in the PM10-treated group. The OVA/PM10 treatment did not produce greater effects than OVA alone. However, the OVA/PM10-treated group did show increased RNA expression of Clca1, Snord22, Retnla, Prg2, Tff2, Atp6v0c-ps2, and Fcgbp when compared to the control groups. These genes are associated with RNA splicing, DNA repair, the inflammatory response, and the immune response. Conclusion: Inhalation of PM10 extensively altered RNA expression while also inducing cellular inflammation, fibrosis, and increased inflammatory cytokines in this murine mouse model.-
dc.formatapplication/pdf-
dc.language영어-
dc.publisherTAEHAN KYORHAEK HYOPHOE-KOREAN ACAD TUBERCULOSIS & RESPIRATORY DISEASES-
dc.relation.isPartOfTUBERCULOSIS AND RESPIRATORY DISEASES-
dc.titleEffects of Particulate Matter 10 Inhalation on Lung Tissue RNA expression in a Murine Model-
dc.typeArticle-
dc.contributor.googleauthorHan, Heejae-
dc.contributor.googleauthorOh, Eun-Yi-
dc.contributor.googleauthorLee, Jae-Hyun-
dc.contributor.googleauthorPark, Jung-Won-
dc.contributor.googleauthorPark, Hye Jung-
dc.identifier.doi10.4046/trd.2020.0107-
dc.relation.journalcodeJ02761-
dc.identifier.eissn2005-6184-
dc.subject.keywordParticulate Matter-
dc.subject.keywordRNA Sequencing-
dc.subject.keywordLung-
dc.contributor.affiliatedAuthorHan, Heejae-
dc.contributor.affiliatedAuthorLee, Jae-Hyun-
dc.contributor.affiliatedAuthorPark, Jung-Won-
dc.contributor.affiliatedAuthorPark, Hye Jung-
dc.identifier.wosid000604777100006-
dc.citation.titleTUBERCULOSIS AND RESPIRATORY DISEASES-
dc.citation.volume84-
dc.citation.number1-
dc.citation.startPage55-
dc.citation.endPage66-
dc.identifier.bibliographicCitationTUBERCULOSIS AND RESPIRATORY DISEASES, Vol.84(1) : 55-66, 2021-01-
dc.identifier.rimsid67598-
dc.type.rimsART-
dc.description.journalClass1-
dc.description.journalClass1-
dc.subject.keywordAuthorParticulate Matter-
dc.subject.keywordAuthorRNA Sequencing-
dc.subject.keywordAuthorLung-
dc.subject.keywordPlusBRONCHIAL EPITHELIAL-CELLS-
dc.subject.keywordPlusNUCLEOTIDE-BINDING DOMAIN-
dc.subject.keywordPlusAIR-POLLUTION-
dc.subject.keywordPlusIMMUNE-RESPONSE-
dc.subject.keywordPlusEXPOSURE-
dc.subject.keywordPlusAMBIENT-
dc.subject.keywordPlusINNATE-
dc.subject.keywordPlusASTHMA-
dc.type.docTypeArticle-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscopus-
dc.relation.journalWebOfScienceCategoryRespiratory System-
dc.relation.journalResearchAreaRespiratory System-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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