Cited 9 times in
Effects of Particulate Matter 10 Inhalation on Lung Tissue RNA expression in a Murine Model
DC Field | Value | Language |
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dc.contributor.author | Han, Heejae | - |
dc.contributor.author | Oh, Eun-Yi | - |
dc.contributor.author | Lee, Jae-Hyun | - |
dc.contributor.author | Park, Jung-Won | - |
dc.contributor.author | Park, Hye Jung | - |
dc.date.accessioned | 2021-03-31T02:10:43Z | - |
dc.date.available | 2021-03-31T02:10:43Z | - |
dc.date.created | 2021-02-22 | - |
dc.date.issued | 2021-01 | - |
dc.identifier.issn | 1738-3536 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/181871 | - |
dc.description.abstract | Background: Particulate matter 10 (PM10; airborne particles <10 mu m) inhalation has been demonstrated to induce airway and lung diseases. In this study, we investigate the effects of PM10 inhalation on RNA expression in lung tissues using a murine model. Methods: Female BALB/c mice were affected with PM10, ovalbumin (OVA), or both OVA and PM10. PM10 was administered intranasally while OVA was both intraperitoneally injected and intranasally administered. Treatments occurred 4 times over a 2-week period. Two days after the final challenges, mice were sacrificed. Full RNA sequencing using lung homogenates was conducted. Results: While PM10 did not induce cell proliferation in bronchoalveolar fluid or lead to airway hyper-responsiveness, it did cause airway inflammation and lung fibrosis. Levels of interleukin 1 beta, tumor necrosis factor-alpha, and transforming growth factor-beta in lung homogenates were significantly elevated in the PM10-treated group, compared to the control group. The PM10 group also showed increased RNA expression of Rn45a, Snord22, Atp6v0c-ps2, Snora28, Snord15b, Snora70, and Mmp12. Generally, genes associated with RNA splicing, DNA repair, the inflammatory response, the immune response, cell death, and apoptotic processes were highly expressed in the PM10-treated group. The OVA/PM10 treatment did not produce greater effects than OVA alone. However, the OVA/PM10-treated group did show increased RNA expression of Clca1, Snord22, Retnla, Prg2, Tff2, Atp6v0c-ps2, and Fcgbp when compared to the control groups. These genes are associated with RNA splicing, DNA repair, the inflammatory response, and the immune response. Conclusion: Inhalation of PM10 extensively altered RNA expression while also inducing cellular inflammation, fibrosis, and increased inflammatory cytokines in this murine mouse model. | - |
dc.format | application/pdf | - |
dc.language | 영어 | - |
dc.publisher | TAEHAN KYORHAEK HYOPHOE-KOREAN ACAD TUBERCULOSIS & RESPIRATORY DISEASES | - |
dc.relation.isPartOf | TUBERCULOSIS AND RESPIRATORY DISEASES | - |
dc.title | Effects of Particulate Matter 10 Inhalation on Lung Tissue RNA expression in a Murine Model | - |
dc.type | Article | - |
dc.contributor.googleauthor | Han, Heejae | - |
dc.contributor.googleauthor | Oh, Eun-Yi | - |
dc.contributor.googleauthor | Lee, Jae-Hyun | - |
dc.contributor.googleauthor | Park, Jung-Won | - |
dc.contributor.googleauthor | Park, Hye Jung | - |
dc.identifier.doi | 10.4046/trd.2020.0107 | - |
dc.relation.journalcode | J02761 | - |
dc.identifier.eissn | 2005-6184 | - |
dc.subject.keyword | Particulate Matter | - |
dc.subject.keyword | RNA Sequencing | - |
dc.subject.keyword | Lung | - |
dc.contributor.affiliatedAuthor | Han, Heejae | - |
dc.contributor.affiliatedAuthor | Lee, Jae-Hyun | - |
dc.contributor.affiliatedAuthor | Park, Jung-Won | - |
dc.contributor.affiliatedAuthor | Park, Hye Jung | - |
dc.identifier.wosid | 000604777100006 | - |
dc.citation.title | TUBERCULOSIS AND RESPIRATORY DISEASES | - |
dc.citation.volume | 84 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 55 | - |
dc.citation.endPage | 66 | - |
dc.identifier.bibliographicCitation | TUBERCULOSIS AND RESPIRATORY DISEASES, Vol.84(1) : 55-66, 2021-01 | - |
dc.identifier.rimsid | 67598 | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordAuthor | Particulate Matter | - |
dc.subject.keywordAuthor | RNA Sequencing | - |
dc.subject.keywordAuthor | Lung | - |
dc.subject.keywordPlus | BRONCHIAL EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | NUCLEOTIDE-BINDING DOMAIN | - |
dc.subject.keywordPlus | AIR-POLLUTION | - |
dc.subject.keywordPlus | IMMUNE-RESPONSE | - |
dc.subject.keywordPlus | EXPOSURE | - |
dc.subject.keywordPlus | AMBIENT | - |
dc.subject.keywordPlus | INNATE | - |
dc.subject.keywordPlus | ASTHMA | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalWebOfScienceCategory | Respiratory System | - |
dc.relation.journalResearchArea | Respiratory System | - |
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