Cited 5 times in
Superior Effect of the Combination of Carbon-Ion Beam Irradiation and 5-Fluorouracil on Colorectal Cancer Stem Cells in vitro and in vivo
DC Field | Value | Language |
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dc.contributor.author | 금웅섭 | - |
dc.date.accessioned | 2021-01-19T08:16:16Z | - |
dc.date.available | 2021-01-19T08:16:16Z | - |
dc.date.issued | 2020-12 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/181541 | - |
dc.description.abstract | Background: The aim of this study was to investigate whether carbon-ion beam irradiation in combination with 5-fluorouracil (5-FU) is superior to carbon-ion beam irradiation alone in targeting colorectal cancer stem-like cells (CSCs). Materials and methods: Human colorectal cancer (CRC) cells, HCT116 and HT29, were treated with carbon-ion beam irradiation alone or in combination with 5-FU. Cell viability assay, colony and spheroid formation assay, apoptotic assay, and quantitative real-time PCR analysis of apoptosis- and autophagy-related gene expression were performed. Results: Carbon-ion beam irradiation dose-dependently decreased CRC cell viability and showed significantly enhanced cell killing effect when combined with 5-FU. Carbon-ion beam irradiation in combination with 5-FU significantly increased the percentage of apoptotic cells. The expression of some apoptotic and autophagy-related genes such as Bax, Bcl2, Beclin1 and ATG7 was significantly induced by carbon-ion beam irradiation alone and was further enhanced when the beam was combined with 5-FU. The spheroid forming capacity of CD133+ cell subpopulations was significantly inhibited by carbon-ion beam in combination with 5-FU. Histopathologically, the combination of carbon-ion beam irradiation and 5-FU destroyed more xenograft tumor cells, and resulted in increased necrosis, cavitation, and fibrosis, compared to carbon-ion beam irradiation alone. Conclusion: In conclusion, carbon-ion beam treatment combined with 5-FU has the potential to kill CRC cells including CSCs by inducing increased apoptosis and autophagy. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Dove Medical Press | - |
dc.relation.isPartOf | ONCOTARGETS AND THERAPY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Superior Effect of the Combination of Carbon-Ion Beam Irradiation and 5-Fluorouracil on Colorectal Cancer Stem Cells in vitro and in vivo | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiation Oncology (방사선종양학교실) | - |
dc.contributor.googleauthor | Woong Sub Koom | - |
dc.contributor.googleauthor | Sei Sai | - |
dc.contributor.googleauthor | Masao Suzuki | - |
dc.contributor.googleauthor | Akira Fujimori | - |
dc.contributor.googleauthor | Shigeru Yamada | - |
dc.contributor.googleauthor | Hirohiko Tsujii | - |
dc.identifier.doi | 10.2147/OTT.S276035 | - |
dc.contributor.localId | A00273 | - |
dc.relation.journalcode | J02422 | - |
dc.identifier.eissn | 1178-6930 | - |
dc.identifier.pmid | 33335403 | - |
dc.subject.keyword | 5-FU | - |
dc.subject.keyword | colorectal cancer stem cell | - |
dc.subject.keyword | heavy ion radiation | - |
dc.contributor.alternativeName | Koom, Woong Sub | - |
dc.contributor.affiliatedAuthor | 금웅섭 | - |
dc.citation.volume | 13 | - |
dc.citation.startPage | 12625 | - |
dc.citation.endPage | 12635 | - |
dc.identifier.bibliographicCitation | ONCOTARGETS AND THERAPY, Vol.13 : 12625-12635, 2020-12 | - |
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