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DNA Methylation of Intragenic CpG Islands are Required for Differentiation from iPSC to NPC

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dc.contributor.author김락균-
dc.date.accessioned2021-01-19T08:06:37Z-
dc.date.available2021-01-19T08:06:37Z-
dc.date.issued2020-12-
dc.identifier.issn2629-3269-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/181467-
dc.description.abstractThe effects of gene body DNA methylation on gene regulation still remains highly controversial. In this study, we generated whole genome bisulfite sequencing (WGBS) data with high sequencing depth in induced pluripotent stem cell (iPSC) and neuronal progentior cell (NPC), and investigated the relationship between DNA methylation changes in CpG islands (CGIs) and corresponding gene expression during NPC differentiation. Interestingly, differentially methylated CGIs were more abundant in intragenic regions compared to promoters and these methylated intragenic CGIs (iCGIs) were associated with neuronal development-related genes. When we compared gene expression level of methylated and unmethylated CGIs in intragenic regions, DNA methylation of iCGI was positively correlated with gene expression in contrast with promoter CGIs (pCGIs). To gain insight into regulatory mechanism mediated by iCGI DNA methylation, we executed motif searching in hypermethylated iCGIs and found NEUROD1 as a hypermethylated iCGI binding transcription factor. This study highlights give rise to possibility of activating role of hypermethylation in iCGIs and involvement of neuronal development related TFs. Graphical Abstract The relationship between iCGI DNA methylation and expression of associated genes in neuronal developmental process. During iPSC to NPCdifferentiation, iCGI containing neural developmental genes show iCGI's DNA hypermethylation which is accompanied by gene activation and NEUROD1which is one of the core neuronal TFs interacts with hypermethylated iCGI regions.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherHumana Press-
dc.relation.isPartOfSTEM CELL REVIEWS AND REPORTS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleDNA Methylation of Intragenic CpG Islands are Required for Differentiation from iPSC to NPC-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentBioMedical Science Institute (의생명과학부)-
dc.contributor.googleauthorWon-Young Choi-
dc.contributor.googleauthorJi-Hyun Hwang-
dc.contributor.googleauthorAnn-Na Cho-
dc.contributor.googleauthorAndrew J Lee-
dc.contributor.googleauthorJungwoo Lee-
dc.contributor.googleauthorInkyung Jung-
dc.contributor.googleauthorSeung-Woo Cho-
dc.contributor.googleauthorLark Kyun Kim-
dc.contributor.googleauthorYoung-Joon Kim-
dc.identifier.doi10.1007/s12015-020-10041-6-
dc.contributor.localIdA04520-
dc.relation.journalcodeJ02682-
dc.identifier.eissn2629-3277-
dc.identifier.pmid32975781-
dc.identifier.urlhttps://link.springer.com/article/10.1007/s12015-020-10041-6-
dc.subject.keywordDNA methylation-
dc.subject.keywordInduced pluripotent stem cell-
dc.subject.keywordIntragenic CpG island-
dc.subject.keywordNEUROD1-
dc.subject.keywordNeuronal progenitor cell-
dc.contributor.alternativeNameKim, Lark Kyun-
dc.contributor.affiliatedAuthor김락균-
dc.citation.volume16-
dc.citation.number6-
dc.citation.startPage1316-
dc.citation.endPage1327-
dc.identifier.bibliographicCitationSTEM CELL REVIEWS AND REPORTS, Vol.16(6) : 1316-1327, 2020-12-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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